renaturation是什么意思   renaturation怎么读

renaturation单词基本解析：

renaturation变化用词：

renaturation英英释义：

renaturation中文词源：

renaturation用法和例句：

renaturation

DNA renaturation :Removing the denaturation factors or in proper condition the denatured DNA (ssDNA) restore native structure (dsDNA) and function.

DNA变性后，生物活性丧失，但一级结构没有改变，所以在一定条件下仍可恢复双螺旋结构。

DNA renaturation

DNA复性作用

Hypochromic effect: The absorbance at 260 nm of a DNA solution decreses when the single strands return into double helix at DNA renaturation.

DNA的变性从开始解链到完全解链，是在一个相当窄的温度范围内完成的。在这一范围内，紫外光吸收值达到最大值的50%时的温度称为DNA的解链温度。

Expression, purification and renaturation of HER-2/neu RLD protein

HER-2/neu配体结合区蛋白的表达、纯化及复性研究

in situ-gel competitive renaturation

IGCR

Keywords Buthus martensi Karsch;BmK AS;expression;inclusion body;denaturation in vitro;renaturation in vitro;

东亚钳蝎;蝎毒活性肽;表达;包含体;体外变性;体外复性;

Renaturation of high concentration lysozyme using gel filtration chromatography assisted by artificial chaperones

人工伴侣与凝胶过滤色谱耦合作用促进高浓度溶菌酶复性研究

Renaturation of lysozyme with assistance of artificial chaperone

人工分子伴侣复性体系辅助溶菌酶复性的研究

Keywords SCF-TPO fusion protein;Inclusion bodies;Purification;Renaturation;

人干细胞因子/血小板生成素融合蛋白;包涵体;纯化;复性;

Keywords Glioma;Single-chain Fv fragment（ScFv）;Fusion protein;Immunotoxin;Recombinant;Pseudomonas exotoxin（PE40）;Inclusion body;Renaturation;

人脑胶质瘤;单链抗体;融合蛋白;免疫毒素;绿脓杆菌外毒素;包涵体;基因重组;复性;

Keywords size exclusion chromatography;lysozyme;renaturation process;aggregate;

体积排阻色谱法;溶菌酶;复性过程;集聚体;

Keywords liquid chromatography;column technique;lysozyme;protein;renaturation;purification;

关键词液相色谱;柱技术;溶菌酶;蛋白质;复性;纯化;

Keywords creatine kinase;conformation;denaturation;renaturation;

关键词肌酸激酶;构象;变性;复性;

in situ-gel competitive renaturation(IGCR)

凝胶原位竞争复性技术

Keywords Vibrio Vulnificus cytolysin;renaturation condition;haemolytic activity;

创伤弧菌溶细胞素;复性条件;溶血活性;

There are many difficulties using tranditional renaturation methods, including low yield and too many steps which cannot be used in industry.

利用传统的方法复性复性率低和步骤繁琐,无法用于大规模生产。最近出现的层析复性方法,具有较强的优势。

Keywords Erythrina trypsin inhibitor;renaturation;chromatography;dialysis;

刺桐胰蛋白酶抑制剂;复性;色谱;透析;

Study on chromatographic renaturation of Erythrina trypsin inhibitor

刺桐胰蛋白酶抑制剂的色谱复性研究

THE AFFECTION OF RENATURATION CONDITIONS ON ACTIVITY OF SINGLE CHAIN ANTIBODY 3H11 FROM INCLUSION BODIES

包含体中单链抗体3H11的复性条件对抗体活性的影响

Renaturation of Recombinant Proteins Produed as Inclusion Bodies in E. Coli

包含体内重组蛋白质的复性

inclusion body' s renaturation

包涵体复性

Recent progress in renaturation of inclusion bodies

包涵体复性研究进展

Inclusion-body protein renaturation is a important procedure in the biotechnology.

包涵体蛋白质复性是生物工程下游技术中的一个重要问题。

denaturation renaturation

变性-复性

solid-phase renaturation

固相复性

Comparing with two commonly protein ren aturation techniques, renaturation on the chromatographic column shows to be mor e superior in speed, recovery and easy to be scaled-up.

在此基础上，对重组链激酶的三种复性方式进行了比较。实验证明，柱上复性快速，高效(回收率达90%以上)，易放大，适用于链激酶生产。

The difficulty on research and industrialization is how to get the more renaturing yield with its 9 pairs disulfide bonds.The molecular biology and renaturation technology of reteplase are reported.

在研究开发和产业化过程中的难点是含有9对二硫键，难以提高复性率，综述了瑞替普酶的分子生物学及变性和复性研究进展。

Renaturation of Recombinant-DNA-Derived Porcine Somatotropin

基因工程猪生长激素复性技术的研究

The renaturation of gene recombinant protein is the most important technique in the field of biotechnology which's many methods have been established.

基因重组蛋白质的复性是当前生物工程领域的重要研究课题。

renaturation DNA

复性作用

renaturation kinetics

复性动力学,复性动态研究

renaturation condition

复性条件

renaturation electrophoresis

复性电泳

renaturation rate

复性速率

renaturation rates

复性速率法

Renaturation of rbIL-2 by dialysis against urea concentration gradient

尿素浓度梯度复性重组牛白细胞介素-2

Chromatography renaturation

层析复性

High efficient expression and renaturation of chimeric Fab were successfully fulfilled, which lay a solid foundation for potential application in clinical treatment of hepatoma.

成功实现了嵌合Fab抗体的高效表达及高效复性 ,为进一步探讨其在肝癌治疗中的应用奠定了基础

Prokaryotic expression and renaturation of engineering chimeric Fab antibody against human hepatoma

抗人肝癌基因工程嵌合Fab抗体的原核表达及复性

Expression of human Fab antibody against keratin in E.coli and its renaturation

抗角蛋白Fab抗体在大肠杆菌中的表达与复性研究

A pair of primers of thrombomodulin in rat for PCR reaction were designed and studied.The PCR reactions were optimized by adjusting the dose of primers or template and the renaturation temperature.

摘要设计了大鼠血栓调节蛋白基因PCR扩增引物，并对引物与模板的加入量及退火温度等进行了研究，优化了PCR反应。

optimum renaturation temperature

最适复性温度

In this paper, techniques, such as Biomarker, SCSU, DNA renaturation experiment, DGGE, TGGE, ARDRA, T-RFLP, SSCP, PAPD and ERIC-PCR, commonly used in this field were introduced.

本文介绍了生物标志物法、SCSU、DNA复性分析、DGGE、TGGE、ARDRA、T-RFLP、SSCP、PAPD和ERIC-PCR 图谱分析等方法在土壤微生物群落结构研究中的应用。

This review summarizes the renaturation techniques, especially the recent developments of refolding.

本文总结了包涵体的相关复性技术，重点介绍重折叠的最新进展情况。

Meantime, the mechanism and applications of molecular chaperone systems in the renaturation of proteins were presented.The artificial molecular chaperone systems were also briefly introduced.

本文针对这一技术，综述了分子伴侣的功能和种类、各种分子伴侣体系的作用原理及在蛋白质复性中的应用情况，并简单介绍了人工分子伴侣。

Keywords ribonuclease inhibitor;fusion expression;renaturation;

核糖核酸酶抑制因子;融合表达;复性;

Fusion Expression and Renaturation of Ribonuclease Inhibitor

核糖核酸酶抑制因子的融合表达与复性

Nucleic acid renaturation

核酸复性

Study on the Mechanism of Adsorption at the Liquid-solid Interface and Renaturation of Lysozyme in Liquid Chromatography

液-固界面的吸附及溶菌酶在液相色谱中复性的应用研究

Keywords Liquid chromatography, protein, Denaturation, Renaturation, Kinetics, Free energy of refolding, Energy barrier, Energy well;

液相色谱;蛋白质;变性;复性;动力学;折叠自由能;能垒;能阱;

Keywords B lymphocyte stimulator;TT;inclusion body;renaturation;animal model;experimental allergic encephalomyelitis （EAE）;rheumatoid arthritis （RA）;

淋巴细胞刺激因子;TT;包涵体;复性;动物模型;实验性变态反应性脑脊髓炎（EAE）;类风湿性关节炎（RA）;

Keywords lysozyme;renaturation;artificial chaperones;gel filtration chromatography;

溶菌酶;复性;人工伴侣;凝胶过滤色谱;

Keywords CSFV E2 gene;E.coli expression;renaturation and purification;

猪瘟病毒;E2基因;大肠杆菌;表达;复性;纯化;

Optimization of Expression Conditions for Reteplase Fusion Protein in Escherichia coli and its Renaturation

瑞替普酶融合蛋白在大肠杆菌中表达条件的优化及其复性

Keywords biochemistry engineering;protein renaturation;ultrafilration;reverse micelle;gel extraction;

生物化学工程;蛋白质复性;超滤;反胶团;凝胶萃取;

Design and Resolution of a Simple-Type Column for Renaturation with Simultaneous Purification of Proteins and Its Application to Lysozyme Refolding

用于变性蛋白质复性及同时纯化的简易型色谱柱的研制及其应用

EFFECT OF GUANIDINE HYDROCHLORIDE ON DENATURATION AND RENATURATION OF HUMAN PLACENTAL ALKALINE PHOSPHATASE

盐酸胍对人胎盘碱性磷酸酶变性与复性的影响

The application of Hofmeister effect in recombinant product inclusion body denaturation and renaturation in biotechnology is discussed in particular.

目前重组蛋白生产过程中包含体的变性和复性问题为上述理论研究提供了广泛的应用空间。

AIM: To study the application of urea gradient size exclusion chromatography (SEC) in the renaturation with simultaneous purification of recombinant human stem cell factor (rhSCF) expressed in E.

目的:用脲梯度体积排阻色谱(SEC)复性并同时纯化在大肠杆菌中高表达的人干细胞因子。

The renaturation of polyphenol oxidase from Tobacco denatured in 6 0 mol/L guanidine hydrochloride(GdnHCl) was investigated.

研究了盐酸胍变性烟草多酚氧化酶后的复性。

dilution renaturation

稀释复性

Renaturation of Recombinant Bovine Prethrombin-2 in vitro from Inclusion Bodies in E. Coli

稀释复性法体外复性重组牛凝血酶原-2包涵体

After purification, the target protein further renaturation 2 - BMP via degeneration dissolving, load dialysis bag.

纯化后的的目的蛋白进一步复性，BMP-2经变性液溶解，装入透析袋内。

After renaturation, AIF protein binded DNA and induced nuclear apoptosis.

经EMSA与Hoechst 33258染色实验证实:获得的AIF蛋白具有良好地与DNA结合并可诱导肿瘤细胞核凋亡的能力,凋亡率为37%。

of total bacterial proteins, and exhibited hemolysis activity after digestion by CNBr and renaturation.

经切割、复性后的防御素具有溶血活性。

The ScFv immunotoxin in inclusion was refolded to form correct structure through denaturation and renaturation showed specific and cytotoxic to gastric cancer cell line MGC803.

经包涵体纯化、变性、复性，使其折叠为正确的空间结构，经检测可与胃癌细胞MGC803特异结合，并有一定的细胞毒性。

After the renaturation of antibody Fab, its binding activity was identified by ELISA and antibody competitive inhibitionassay in vitro.

经超声破碎、洗涤、变性和复性后，采用双抗体夹心ELISA法及体外抗体竞争抑制试验鉴定表达产物的生物学活性。

Keywords Hepatocyte growth factor;Inclusion bodies;Protein renaturation;Rats;

肝细胞生长因子;包涵体;蛋白质复性;大鼠;

A comparison of the activity and conformational changes during renaturation of urea--denaturation creatine kinase from rabbit brain

脲变性兔脑肌酸激酶在复性过程中活力与构象变化的比较

Study on Aggregate for Denatured Lysozyme Renaturation in Urea and Guanidine Hydrochloride Solutions

脲或盐酸胍溶液中变性溶菌酶复性过程中集聚体的研究

protein renaturation

蛋白再生

Mobile phase composition is one of the most important factors affecting the efficiency of protein renaturation in PFLC.

蛋白折叠液相色谱法(PFLC)用于变性蛋白质复性并同时纯化时对流动相组成及其洗脱条件的要求远较通常的液相色谱法高。

The protein refolding assisted by the molecular chaperone systems is promised to improve the protein renaturation efficiency.

蛋白质复性，即如何高效地将无活性的包涵体转变成为有活性的蛋白质，已成为基因工程蛋白产业化的瓶颈。

The denaturation and renaturation of proteins is one of those basic problems.

蛋白质的变性、复性问题是与Hofmeister效应密切相关的生化基础课题。

The fusion protein level,renaturation of inclusion bodies,purity of aprotinin,total recovery and the activity were about 45%,70%,90%,32% and 3.1 EPU/mg respectively.

融合蛋白的表达量为45%,包涵体复性率为70%以上,抑肽酶纯度为90%,总收率为32%,比活力为3.1 EPU/mg。

Keywords angiostatin;fermentation;optimization;Orthogonal Design;renaturation;

血管抑素;发酵;优化;正交试验;复性;

THE EFFECTS OF HYDROPHOBICITY AND CHARGE OF SURFACTANTS ON THE ACTIVITY AND RENATURATION ABILITY OF CREATINE KINASE

表面活性剂的疏水性及电性对肌酸激酶的活力及复性能力的影响

Keywords retinol dehydrogenase/reductase;alternative splicing;renaturation;activity;

视黄醇脱氢/还原酶;选择性剪接;复性;活性;

Keywords superoxide dismutase;surfactants;degeneration;renaturation;

超氧化物歧化酶;表面活性剂;变性;复性;

dialysis renaturation

透析复性