transfect是什么意思   transfect怎么读

transfect单词基本解析：

transfect变化用词：

transfect英英释义：

transfect中文词源：

transfect用法和例句：

transfect

3) Purified recombinant Ad plasmid DNA is digested with PacI to expose its inverted terminal repests( ITR), and then used to transfect AD-293 cells where deleted viral assembly genes are complemented in vivo.

3) 用线性化的重组腺病毒转染293细胞，获得基因组结构均一的重组腺病毒。

AD5/F35 and AD5 vectors could not effectively transfect cells of T cell origin, no fluorescence positive cells were detected at MOI=1000;

AD5/F35和AD5载体都不能有效地感染T系来源细胞，在MOI达到1000也未检测到荧光阳性细胞。

VEGF gene transfect

VEGF转染

The gene transfection experiment in vitro showed that the pDNA-chitosan nanoparticles can transfect BHK celles , and the gene can express in these celles.

体外基因转染实验表明壳聚糖作为一种新型的非病毒基因递送载体能够高效传递DNA进入BHK-21细胞，基因能够在该细胞中高效表达；

Transfect PK-15 cells with SK-PCV2-1(DB) in vitro. After four passages,the infectivity of the cloned chimeric PCV2-1 genome was confirmed by RT-PCR and IFA.

体外转染PK-15细胞,盲传4代后,用RT-PCR和间接免疫荧光方法检测,结果表明本试验构建的嵌合病毒PCV2-1克隆具有感染性。

Keywords antisense-VEGF;gene transfect;cationic liposome;laryngeal cancer;

关键词反义VEGF;基因转染;阳离子脂质体;喉癌;

Keywords retinal pigment epithelial cells;cell culture;ricombinant insulin-like growth factor;transfect;cells proliferation;

关键词视网膜色素上皮细胞;细胞培养;重组胰岛素样生长因子;转染;细胞增殖;

Keywords Meniscus;Cell;Human insulin-like growth factor（hIGF）;Gene;Transfect;

半月板;细胞;胰岛素样生长因子;基因;转染;

Polymeric carrier containing folic acid is expected to be able to identify tumor surface receptors and transfect cells by receptor-mediated endocytosis.

含有叶酸的聚合载体能够识别肿瘤表面受体，并且能够通过受体介导的(细胞)内摄作用转染细胞。

The aim of gene therapy is to transfect DNA into target cells, in which ultilizing antisense oligonucleotides is a main method.

基因治疗是将DNA转染进入目的细胞,修复遗传错误或产生治疗因子。反义寡核苷酸的应用是基因治疗的主要手段之一。

gene transfect

基因转染

Gene transfection is a technology that heterologous genes transfect eukaryotic cells, this article will summarize the significance, classification, methods and application of transfection.

基因转染技术是将外源基因转染真核细胞的一种技术,本文将对外源基因转染真核细胞的目的意义、转染技术分类、方法及其应用等做一综述。

Adenovirus-mediated Rb Gene Transfect for Head and Neck Cancer

外源性Rb基因转染对头颈肿瘤的抑制作用

Keywords colorectal,heparanase,metastasis,basement membrane protein,transfect,anti-sense olgodexynucleotide（ASODN）,implantation of histologically intact,laminarin;

大肠癌;乙酰肝素酶;转移;基膜蛋白;转染;成瘤性;原位接种;昆布多糖;

These clones were used to transfect fetal donkey dermal (FDD) cells and donkey differentiated monocyte-macrophages (DL), and their infectious characteristics were monitored by RT-PCR and reverse transcriptase activity assay.

将所改造的突变克隆转染驴胎皮肤细胞(FDD)以及驴单核巨噬细胞(DL),并用逆转录酶活性检测和 RT PCR方法验证其感染性。

The recovered bands of PrP, GFP and pCDNA3.1(-) were ligated with T4 ligase to produce a fusion expression plasmid, pCDNA3.1(-)-mPG, which was then used to transfect the Neuro-2a cell under the selection pressure of G418 antibiotics.

将这三个片段共连接,从而获得融合mPrP/GFP基因的真核表达质粒pCDNA3.1(-)-mPG。 由于在PrP C-端融合了绿色荧光蛋白(GFP),使该系统具备了早期筛选的标签。

Keywords Toxoplasma gondii;P22;gene transfect;expression;macrophage;

弓形虫;P22;基因转染;基因表达;巨噬细胞;

Methods:NPC cell line (CNE1) as target,Electroporation was used to transfect EBV-LMP gene and the vector into CNE1 cells.

方法：以人高分化鼻咽癌细胞株(CNE1)为对象，采用电穿孔基因转染技术，将重组EBV-LMP表达质粒转染CNE1细胞。

This context concludes many researches about ultrasound microbubble: a novel gene vector in recent years.These researches include functional mechanism, influence factor, transfect...

本文对近年来有关超声微泡造影剂作为基因转染载体的作用机制、影响因素、转染实验和应用等方面的研究作了总结。

The constructed wild type p21 gene promoter driven luciferase repo rter plasmid is used to transfect ECV304 cells to express luciferase protein sta bly and reliably.

构建的野生型p21基因启动子驱动的荧光素酶报告基因载体,转染ECV304细胞后能够表达荧光素酶蛋白,具有稳定和可靠性能。

Keywords Human dendritic cells;MUC1/Y;CTL;eukaryotic expression vector;liposomes;transfect;ovarian cancer;biotherapy;

树突状细胞;细胞毒T淋巴细胞;真核表达载体;真核细胞;脂质体;转染;卵巢癌;MUC1/Y;生物治疗;

We transfect the recombinant MEK gene into human umbilic vein endothelial cells ECV304, then examine the change of ESM-1 expression in cells transfected with recombinant MEK gene of differenceactivity .

用重组MEK基因转染人脐静脉内皮细胞ECV304，加以鉴定，并检测转染细胞中ESM-1表达的改变。

Electric transfect

电转染

Objective: The aim was to construct the recombinant of neuro related cell adhesion molecule(NrCAM) and immunoglobin Fc fragment and transfect it into insect cells.

目的 :构建神经原相关的细胞粘附分子 (NrCAM )和免疫球蛋白Fc片段融合基因的重组质粒并通过Baculovirus载体转染到昆虫细胞。

Objective To construct endothelial cell-specific molecular-1(ESM-1) eukaryotic express vector,transfect it into human umbilical vein endothelial cells( HUVECs,ECV304) and express it in vitro .

目的 构建人内皮细胞特异性分子 1(ESM 1)真核表达载体 ,转染人脐静脉内皮细胞系ECV30 4 ,并在ECV30 4中获得表达。

Objective To construct recombinant retroviral vector expressing antisense ANG and transfect it into the human lung cancer cell line A549 to inhibit the expression of ANG.

目的 构建含反义人血管生成素的真核表达载体 ,并转染人肺癌细胞株A5 4 9,使之特异性地封闭A5 4 9细胞中血管生成素的表达 ,以达到抑制肿瘤生长的目的。

AIM: To clone human IL-24 (hIL-24) gene and construct its eukaryotic expression vector, then transfect it into Ca Ski cells to express hIL-24 protein.

目的:克隆人IL-24基因并构建真核表达载体,转染Ca Ski细胞进行真核表达.

Objective: To construct eukaryotic expression vector pSecTag2/HygroB-DAF of human decay accelerating factor (DAF) and transfect NIH/3T3 cells after encapsulated by chitosan.

目的:构建人补体衰变加速因子(DAF)真核表达载体pSecTag2/HygroBDAF,并利用壳聚糖组装成纳米微粒复合体,转染小鼠NIH/3T3成纤维细胞。

Objective To transfect pcDNA3.1/hTM plasmids containing human thrombomodulin (hTM) gene into human umbilical vein endothelial cells (HUVECs),and investigate the expression of hTM and anticoagulating function of transfected HUVECs.

目的将含入凝血酶调节蛋白(hTM)基因的真核表达载体质粒 pcDNA3.1/hTM 转染体外培养的脐静脉内皮细胞(HUVECs),观察外源凝血酶调节蛋白的表达及其所致的 HUVECs 抗凝功能的改变。

Objective To explore the effects of antisense epidermal growth factor receptor(EGF-R)oligodeoxynucleotides on ultraviolet-induced c-jun activity of keratinocytes after EGF-R oligodeoxynucleotides transfect to HaCaT in vitro.

目的探讨表皮生长因子受体(EGF-R)反义寡核苷酸转染体外培养的表皮角质形成细胞株HaCaT后,对紫外线诱导的表皮角质形成细胞转录因子c-jun活性的影响。

Objective To construct recombinant human bone morphogenetic protein 2 (rhBMP2) eukaryotic expressing vector, and to transfect human fibroblasts and other two cell types, and to determine the transient expression of BMP2 in these cells.

目的构建人骨形成蛋白2(BMP2)的真核表达载体以探讨成纤维细胞表达BMP2的情况。

Objective To construct the recombinant plasmid of eukaryotic expression vector containing M-CSF gene and transfect it into the NIH3T3 cells to study whether resulting in the expression of M-CSF and EGFP fusion proteins.

目的构建真核表达重组体pEGFP-M-CSF,并鉴定其在小鼠成纤维细胞NIH3T3细胞能否正确表达。

Keywords nm23-H1;PGBE1;Eukaryotic expression vector;Transfect;

真核表达载体;转染;

Keywords Neurotrophin 3;Gene transfect;Cochlea;Gentamicin;

神经营养因子3;基因转染;耳蜗;庆大霉素;

Conclusion: jetPEI-RGD can efficiently transfect ASODN for inhibiting proliferation effect and inducing apoptosis on caco-2 cells in vitro.

结论：jetPEI-RGD介导的bcl-2反义核酸能抑制caco-2细胞增殖和诱导细胞凋亡。

Keywords Biliary Tract Carcinoma Cell Line;Tumor Suppessor Gene;Transfect;

胆管癌细胞;抑癌基因;转染;

Keywords Adenovirus Transfect 293 cell Ovarian neoplasms Drug resistance;

腺病毒;转染;293细胞;卵巢肿瘤;抗药性;

Keywords membrane proteins;cell proliferation;cell adhesion;gene transfect;

膜白质类;细胞增殖;细胞黏附;基因转染;

membrane proteins; cell proliferation; cell adhesion; gene transfect

膜白质类；细胞增殖；细胞黏附；基因转染

Keywords vascular endothelial growth factor-C;transfect;gene expression;vector;lymphedema;

血管内皮生长因子-C;转染;基因表达;载体;淋巴水肿;

Transfect ion

转染

transfect methods

转染方法

The chemotaxis and calcium mobil i zation assay were used for examining bioactivities of the cells stably transfect ed with CCR4.

通过基因转染技术获得稳定转染细胞株，利用趋化实验、钙流实验证实稳定转染细胞株能有效表达CCR4并具有生物功能。

Lipofectamine was used to transfect the carcinoma of gallbladder.The inhibition of telomerase activity and cell proliferation in GBC-SD cell was observed, and compared with control group.

酶切及测序鉴定正确后，采用脂质转染法导入胆囊癌细胞,观察其对胆囊癌细胞端粒酶活性的影响及对细胞生长、增殖的作用，并设空载体转染组和单纯脂质体转染组作为对照。

Keywords mesenchymal stem cells;bone morphogenetic protein12;committed differentiation;tenocytes;tissueengineering;seed cells;gene transfect;Western Blotting;flow cytometry;

间充质干细胞;骨形态发生蛋白12;定向分化;健细胞;组织工程;种子细胞;基因转染;免疫印迹;流式细胞术;

Cationic liposome mediated NT-3 gene to transfect mice spiral ganglion cells

阳离子脂质体介导小鼠原代螺旋神经节细胞NT-3基因转染的实验研究

Expression of D1x5 mRNA disapears at the fourth passage NSCs,but can be recovered by exogenous gene transfect ion and treatment with BMP-2. In the meantime, D1x5 gene can direct in vitro expanded SVZa NSCs differentiating into neurons.

随着传代次数的增加,到第4代时,SVZa NSCs D1x5 mRNA表达消失,而外源性D1x5 基因转染及BMP-2可以诱导其重新表达; D1x5基因能使新生大鼠来源的SVZa NSCs向神经元方向分化。

Transfect of Exogenous in Vivo of Chickens Germinal Disc

鸡胚盘内转染外源基因的研究