supernatant单词基本解析：

adj.浮在表面的
adj.浮在表面的, [化]上层清液
adj.浮在表面的；

supernatant变化用词：

副词: supernally |

supernatant英英释义：

Noun

1. a liquid lying above a sediment (floating on the surface)

名词 supernatant:

the clear liquid that lies above a sediment or precipitate

形容词 supernatant:

of a liquid; floating on the surface above a sediment or precipitate

supernatant[ ,sju:pə'neitənt ]n.the clear liquid that lies above a sediment or precipitateadj.of a liquid; floating on the surface above a sediment or precipitate

"the supernatant fat was skimmed off"

supernatant中文词源：

supernatant用法和例句：

The culture supernatant of tumor cell lines was not cytoxic.

白血病细胞培养上清液无细胞毒作用.

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Methods Manner plate assay, contact hemolysis and supernatant assay were used.

方法平板检测法, 接触溶血法,上清检测法.

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You centrifuge the solution, decant the supernatant, and discard the pellet.

你然后对溶液离心, 慢慢倒出上清,弃沉淀.对上清液你加更多的硫酸铵.

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Once precipitated the sample was centrifuged and the supernatant was removed.

如有沉淀,离心样品,出去上层漂浮物.

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Transfer the supernatant into a spin ( QIAprep ) column.

将上清液换至( Qiaprep ) 离心管柱.

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The supernatant fat was skimmed off.

浮在表面的肥油已经被撇去了.

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Objective : To study the growth factor like action of the Sika Deer splenocytes culture supernatant.

目的: 测定梅花鹿脾细胞培养上清的生长因子样活性.

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Many other proteins in the supernatant could interfere the binding of destined protein.

裂解上清杂蛋白浓度较高会干扰目的蛋白的亲和纯化.

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Objective To investigate the carcinogenetic and tumor promoting effect of concentrated Helicobacter pylori culture supernatant ( CHCS ).

目的观察大鼠胃粘膜在幽门螺杆菌 ( Hp ) 培养滤液长期作用下的组织学变化及其对大鼠实验性胃癌的促癌作用.

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The 24 - hour culture supernatant of AM could facilitate the FB proliferation and the TGF - � � _ 1 expression.

AM的24小时培养上清有促进FB增殖及转化生长因子 - β1表达的作用.

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supernatant

GT1-7 cells were incubated with galanin at a cohort of concentrations for different time points and GnRH released in the supernatant was determined by RIA.

(2 )将不同浓度的甘丙肽以不同时间与GT1- 7细胞孵育 ,用RIA法测定细胞上清液中GnRH含量。

(2) The supernatant of C6 glioma cells have the signal substance which can induce migration of NSCs in vitro.

(2)C6胶质瘤细胞无血清培养的上清中存在着能够诱导神经干细胞迁移的物质。

The splenocytes stimulated with supernatant from syngeneic or allogeneic MLTC of H22 tumor cells generated lower proportion of CD4+CD25+ T cells than that of D5 tumor cells（p<0.05）.

D5刺激的同系脾细胞上清培养的脾细胞中的CD4+CD25+T细胞与同种脾细胞上清相比明显增多（p<0.001）,与H22刺激的同系或同种脾细胞上清相比，也能诱导更多的CD4+CD25+T细胞（p<0.05）。

Compared with normal MsC, the number of G2-M cells treated with DCN-containing supernatant decreased to 35%(P<0.05), while the expression of phospho-ERK1/2 and phospho-SAPK/JNK on MsC was enhanced significantly (P < 0.05).

DCN上清明显抑制正常MsC的增殖,G2-M期细胞数明显减少,仅为对照组的35%(P<0.05);

Keywords EM bacteria material;garbage supernatant;active soil;bacteria membrane;

EM菌剂;垃圾渗沥液;活性污泥;生物膜;

The elevation of MDA content of hepatocytes and LDH level in supernatant of hepatocytes induced by CP were restored remarkably by HSS.

HSS明显降低由CP升高的肝细胞培养上清液中LDH水平和肝细胞MDA含量。

The IL-6 secretion and IL-6 mRNA expression were significantly increased in FLS cultured with the supernatant from U937 cell treated with LPS.

LPS刺激的U937细胞培养液上清液可明显增强FLS中IL 6蛋白分泌及mRNA表达 ;

The endothelial cells incubated with the supernatant of MBP-stimulated PBMC can promote T cell proliferation

MBP刺激的PBMC培养上清对内皮细胞促进T细胞增殖的作用

MaAb culture supernatant

McAb培养上清

Effects of MSB 1 Culture Supernatant to MDV

MSB-1培养物上清液对MDV的作用

The mIL 12 in the supernatant was detected by ELISA after the pCmIL 12 was transfected into COS 7 cells.

p Cm IL - 12在体外转染 COS- 7细胞后 ,经 EL ISA证实有 m IL- 12表达 ,其表达上清能在体外明显增强小鼠 NK细胞活性。

Quantibody arrays can be used in cell culture supernatant, serum, plasma, CSF, tissue lysate, and other body fluid.

Quantibody芯片可用于培养细胞上清、血清、血浆、集落刺激因子、组织匀浆以及其他生物学液体。

RT PCR result showed that after 4h,12h,24h incubation respectively, 30% supernatant can not increased insulin messenger RNA levels significantly at the present of 16.7mmol/L glucose compared with INS 1 cells cultured with glucose alone.

RT PCR结果显示 ,在 1 6 7mmol L葡萄糖刺激 4、1 2和 2 4h后 ,30%上清液对INS 1细胞胰岛素mRNA水平均无明显影响。

Study on Antitumor Activities in Supernatant Preparations from T-AK Cells in Culture

T-AK细胞上清液抑瘤活性的研究

The culture supernatant(SN)of T6.6could augment NK activity significantly and the augmen-tation was more efficient on combination with IL-2.Theaugmentation could be blocked by anti-IL- 6 MoAb.

T6.6培养上清能显著增强自然杀伤（NK）活性，当与IL－2合用时增强效果更佳，抗IL－6单抗能阻断这种增强作用。

The supernatant was collected for HCG assay. [Results] Ectopic HCG increased in EAC and JEC with prolonged culture period, especially in JEC.

[结果]随着培养时间的延长,两株细胞自分泌的HCG量有所增加,特别JEC株表现更加明显。

supernatant town is a millennium-old ancient town, located in the scenic Shangqing Gong, 25 km away from the urban Yingtan.

上清古镇是一个具有千年历史的古镇，位于上清宫景区内，距鹰潭市区25公里。

After enzymatic hydrolysis,the yield of XOS could be up to 84.93% of total carbohydrates of the supernatant.

上清液经木聚糖酶酶解后,低聚木糖的含量可达到上清液总糖的84.93%。

TES could maitain 85 cell"s subsistence.Maybe supernatant affect growth cycle of T cell line.

上清液能维持85细胞生存，可能与影响T细胞生长周期有关。

The results show that when PAC was used as the sole coagulant, supernatant with low turbidity can be achieved, however, only at very high dosage.

中文摘要台湾地区因具高山陡峻、河川短促之地理特性，河川流速湍急，河岸冲刷严重，且洪、枯水量差异大；

Study on the Influence of Immunosuppression of Supernatant of S180 by AMB and PUPS

中药黄芪注射液、猪苓多糖注射液影响S180肿瘤细胞培养上清免疫抑制作用的研究

In this study,the gene of DCD-1L,a derivant of Dermcidin,was first cloned into plasmid pPIC9 and expressed in Pichia pastiros GS115.The supernatant extracted from the expressing system had the activities against E.

为了能快速并低成本地获得该肽 ,DCD 1L基因 ,第一次被克隆到毕赤酵母载体pPIC9中 ,并在毕赤酵母GS1 1 5中进行表达。

breast carcinoma supernatant

乳癌上清液

AN AGGLUTINATE SUBSTANCE IN THE SUPERNATANT OF HUMAN MYELOID LEUKEMIC CELLS(HL-60)

人早幼粒白血病细胞hl_(60)能够产生具有凝集活性的物质

The test status of calcium removal of carbide slag supernatant and sulfur and phosphorus removal of waste water was introduced.

介绍了电石渣上清液除钙及工艺废水除硫、磷的试验工作。

However, Shenzhen supernatant drink in the team after getting over by Li Jianhua through a plug out of the ball, Johnson missed long-range De Qiuhou.

但是深圳上清饮队在缓过劲后，由李建华塞出了一个穿越球，约翰森得球后远射偏出。

Submandibular tissues of rats were incubated in vitro and different concentration of FSH was added.Enzyme link immunoassay was used to measure EGF and NGF in supernatant.

体外孵育大鼠下颌下腺组织并给予不同浓度FSH,应用酶联免疫分析方法检测上清液中NGF和EGF的含量。

You centrifuge the solution, decant the supernatant, and discard the pellet.

你然后对溶液离心，慢慢倒出上清，弃沉淀。对上清液你加更多的硫酸铵。

You proceed with the purification using the supernatant fraction that contains mostly intact mitochondria.

你继续用含大多为完整的线粒体的上清液纯化。

Using the saliva supernatant, they isolated RNA, performed two-round linear amplification with T7 RNA polymerase, and applied human genome U133A microarrays for profiling human salivary transcriptome.

使用唾液离心后的上清液，他们分离出RNA，以T7 RNA聚合脢进行两个回合的线性放大作用，以及应用人类基因体U133A微阵列来分析人类唾液转录体。

Keywords Lactic acid bacteria;supernatant;human pathogens;inhibitory action;

关键词乳酸菌;上清液;人源肠道致病菌;抑菌特性;

Keywords marrow stromal cells;supernatant;umbilical cord blood;hemapoietic cells;expand in vitro;suface markers;

关键词骨髓基质细胞;上清;脐血;造血细胞;体外扩增表面标志;

Low Radioactivity Used in Night Soil Supernatant Treatment

利用低放射性处理粪便上清液技术

Keywords homogenate supernatant;dentritic cells;endothelialization;

匀浆上清;树突状细胞;内皮化;

Keywords Cordyceps militaris;Fermentation supernatant;Cordycepin;Adenosine;Analysis by HPLC;

北冬虫夏草;发酵液;虫草素;腺苷;HPLC分析;

anaerobic phosphate-enriched supernatant

厌氧富磷上清液

Keywords BNR;anaerobic supernatant;model;phosphorus recovery;strippant;struvite;

厌氧池上清液;模型;磷回收;分离液;鸟粪石;

Bifidobacterium-cultivated supernatant

双歧杆菌培养上清

Retrovirus mediated gene transfection was carried out. First, the package cell line PT67 was transfected by lipofectAMINE TM2000, so as to obtain recombinant retrovirus supernatant, which was directly used in the transfection of MSCs.

反转录病毒介导的基因转染,首先利用LipofectAMINETM 2000转染包装细胞系PT67,获得重组反转录病毒上清,然后用病毒上清直接感染骨髓间充质干细胞。

Spent culture supernatant(SCS)

发酵上清液

The supernatant was examined by SDS-PAGE protein electrophoresis .

取上清进行SDS-PAGE蛋白质电泳。

Take 150 ml of supernatant for pH check and SCOD and sulfate analysis.

取出150ml上清液测定pH、SCOD和硫酸盐。

In addition, some culture media supernatant of directly irradiated cells were transferred to the non-irradiated cells culture medium, forming the group P2.

另将直接受照细胞培养液转移至未受照细胞培养瓶中形成旁效应2组。

Keywords olfactory ensheathing cells（OECs）;supernatant;glial cell line-derived neurotrophic factor （GDNF）;content;

嗅鞘细胞;上清;胶质源性神经生长因子（GDNF）;含量;

Keywords Lactobacillus acidophilus;Spent culture supernatant;Antibiotic-associated diarrhoea;Intestinal flora;

嗜酸性乳杆菌;耗尽培养上清液;抗生素相关性腹泻;肠道菌群;

Keywords Lactobacillus acidophilus;spent culture supernatant （SCS）;diarrhea;

嗜酸性乳杆菌;耗尽培养上清液;腹泻;

Effects of spent culture supernatant of Lactobacillus acidophilus on intestinal flora in mice with antibiotic-associated diarrhoea

嗜酸性乳杆菌培养上清液对抗生素相关性腹泻小鼠肠道菌群的影响

It was found that the fixed cell of NIH3T3/TM TNFm, the supematant of NIH3T3/S TNFm, both fixed cell and supernatant of NIH3T3/Wt TNF could effectively kill tumor cell H22 in vitro.

在体外 ,NIH3T3/TM TNFm的固定细胞、NIH3T3/S TNFm的上清、NIH3T3/Wt TNF的固定细胞和上清均可有效杀伤肿瘤细胞H2 2。

The cytopathic effect(CPE),beating frequency of myocardial cells and the lactate dehydrogenase(LDH)levels in supernatant was tested at day 2,3,5 respectively after the treatment.

在处理的d 2,3,5观察各组细胞病变效应(CPE)、细胞搏动频率并测定细胞上清液中乳酸脱氢酶浓度。

when growing in liquid culture,its supernatant fluid was clear with granular sediment in it and the sediment couldn't disperse completely after shaking.

在液体培养基中,上清液清亮并有颗粒状沉淀,摇动沉淀不能完全散开。除此之外,该菌的其它特性均符合典型大肠杆菌的特征。

cultural supernatant

培养上清

In addition, BPF and GM-CSF could also be found in the supernatant collected on 24th day of culture.

培养第24天的上清液中还可检出BPF和GM-CSF活性。

GTCC application on treatment for supernatant layer of vinasse of corn alcohol

壳聚糖季铵盐处理玉米酒精清糟液的研究

The study shows the rhein can appreciably increase the survival rate of acinar cell and lower the levels of LDH,AMY and NAG in the supernatant.

大黄酸可提高相应肿瘤坏死因子浓度下的胰腺细胞的细胞活率,降低培养上清中 LDH、AMY 和 NAG 的水平。

The clear supernatant of rat macrophage cultivated in DMEM culture fluid containing 12.5 mol/L glucose could markedly promote the hyperplasia of mesangial cell(P<0.01).

大鼠巨噬细胞在含12.5mol/L葡萄糖的DMEM培养液中培养24h,其上清明显促进系膜细胞增殖的(P<0.01)。

Isolation of rat hepatocytes and effect of BRL cell culture supernatant on rat hepatocytes proliferation

大鼠肝细胞的分离及BRL细胞培养上清对原代肝细胞增殖的影响

Once precipitated, the sample was centrifuged and the supernatant was removed.

如有沉淀，离心样品，出去上层漂浮物。

Effect of sixpetal clematis root parenteral solution on interlukin-1 level in osteoarthritis joint fluid and chondrocyte culture supernatant

威灵仙注射液对骨关节炎关节液与软骨白介素-1水平的影响

The anisotropic of the supernatant was smaller than that of the bottom layer.

存放还引起粘度下降，估计这与液晶生成会使粘度下降有关。

Experimental grouping is as follows: group A: DC+T+SMMC-7721 supernatant;

实验分组如下：A组: DC+T+ SMMC7721上清；B组: DC+T。

SDS-PAGE results indicated that there was a clear target band in Muts and Mut+ recombinant Culture supernatant after 48 hours culture respectively.

实验结果表明,诱导表达48h后,Mut+和MutS重组子表达的产物在SDS-PAGE胶上都出现了清晰的目的带。

Two inactivated vaccine with different antigen concentration were prepared by using the supernatant materials obtained from centrifuging the ground emulsion of SPF whole embryos infected with AEV Van Rockel strain.

将感染了AEV－VanRockel株的SPF全胚磨制的乳剂离心分离，取上清液制成抗原含量不同的两种灭活疫苗。

Carefully transfer the supernatant of the flow-through to a new microcentrifuge tube (not supplied) without disturbing the cell-debris pellet in the collection tube.

小心将液体的上清移至一新的微量离心管中，不要搅动收集管中的细胞残渣沉淀。

Effect of Helicobacter pylori broth culture supernatant on cell proliferation in rat gastric mucosa

幽门螺杆菌培养上清液对大鼠胃黏膜上皮细胞增殖的影响

The carcinogenetic and tumor-promoting effects of concentrated Helicobacter pylori culture supernatant in rats.

幽门螺杆菌培养滤液诱癌和促癌作用的实验研究。

Results COS?7 cells transfected with constructed PCIneo?hTPO expressed and secreted hTPO up to 48.28 ng/ml in the supernatant.

应用hTPOcDNA转染的COS－7细胞表达产物对实验性小鼠血小板减少症进行腹腔注射。 结果hTPOcDNA不仅可在COS－7细胞中获得表达，且分泌到细胞培养上清中，最高表达量为48.28ng/ml。

The recombinant protein, in the culture supernatant, designated C2-45 (IgG1), was purified by affinity chromatography, and characterized for the CEA binding specificity and the biological activity of CDC and ADCC.

应用免疫亲和层析法,纯化培养上清中表达的重组蛋白C245(IgG1),并鉴定其与CEA结合的能力和CH的生物学功能CDC及ADCC。

cardiac muscle lysate supernatant

心肌上清液

Neutrophil chemotactic activity in sputum supernatant was higher than the pool of normal subjects both in chronic asthma and COPD patients.

慢性哮喘患者和COPD患者痰液上清液中的中性粒细胞趋化活性均较正常人高。

ERP-SBR BIO-CHEMICAL PROCESS FOR NITROGEN AND PHOSPHORUS REMOVAL BY DRAINING OUT ANAEROBIC RICH PHOSPHATE SUPERNATANT

排出厌氧富磷污水生物化学除磷脱氮ERP-SBR系统研究

AP has caused widespread concern in the Derby, Guangdong, Shenzhen and drink supernatant team's 0-1 win against Guangzhou Shayuergui medical team.

搜狐体育讯引起广泛关注的广东德比中，深圳上清饮队以0-1的比分负于广州医药队铩羽而归。

In addition to residual turbidity in the supernatant, the zeta potential of the floc formed and the volume of sludge were also monitored.

故后续之研究可考量添加助凝剂于浓缩槽，或将滤床反冲洗水与沉淀污泥分开处理。

The fluorescence intensity of the supernatant was measured by spectrofluorometer. The data were described by two types of binding sites model,the binding parameters of BSM-R8 were obtained by Scatchard plots.

数据符合两种不同结合位点的模型,经Scatchardplots处理后得到R8与BSM的结合参数。

Methods The LDH content in supernatant of endothelial cells after preservation-rewarming to determine the injury after preservation-rewarming.

方法 1. 测定内皮细胞经保存-复温后上清中乳酸脱氢酶（LDH）浓度，比较保存-复温后损伤；

Methods Expression vector pCTLA 4/Ig was transfected into COS 7 with lipid reagent. The expression of fusion protein in the supernatant of the cell culture media was detected with double antibody sandwich ELISA.

方法 将pCTLA 4/Ig表达质粒转染COS 7细胞 ,双抗体夹心ELISA检测细胞培养上清中融合蛋白表达 ;

Methods Mouse IL-3 cDNA was cloned to retrovirus vector pLXSN and then the recombinant IL-3 cDNA was transfected on PA317 cells with lipofectin. The cells was selected with C418 after BMS was transfected by the supernatant containing virus.

方法 将小鼠IL－3cDNA克隆到逆转录病毒载体（pLX－SN）上，然后将重组的IL－3 cDNA用脂质体转染到包装细胞PA317上，G418筛选后得到抗性克隆，将含病毒的上清液转染骨髓基质细胞。

FL in the supernatant was detected by ELISA. MTT was adopted to determine the sensitivity of MCF 7/TK FL cells to GCV (ganciclovir).

方法 构建以IRES(内部核糖体进入位点 )相连的TK与FL的真核表达质粒 ,脂质体法转入人乳腺癌细胞株MCF 7中。

Methods The inhibition activity on P.gingivalis,P.intermedia and F.nucleatum of S.sanguis suspension and supernatant were measured by Pucci test and Agarose gel diffusion method.

方法分别采用交叉双层培养基法和微量琼脂糖弥散法测定血链球菌全菌细胞和培养上清液对牙龈卟啉单胞菌、中间普雷沃菌、具核梭杆菌的拮抗作用。

Methods Peripheral blood mononuclear cells(PBMC) were cultured with different concentration of tumor cell culture supernatant.

方法将不同浓度肿瘤细胞培养上清液与体外分离的人淋巴细胞一起培养;

Methods: The culture supernatant of cord blood LAK cells and diluted E.coli suspension were cocultured in agar medium and the colonies of E.coli were counted.

方法将脐血LAK细胞培养上清液和稀释的埃希氏大肠杆菌悬液混合,在培养皿内培养不同时间,计数各个培养皿内细菌菌落数。

用作形容词(adj.)

The supernatant fat was skimmed off.
浮在表面的肥肉已经被撇去了。

You centrifuge the solution, decant the supernatant, and discard the pellet.
你然后对溶液离心，慢慢倒出上清，弃沉淀。对上清液你加更多的硫酸铵。

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