antisense是什么意思   antisense怎么读

antisense单词基本解析：

antisense变化用词：

antisense英英释义：

antisense中文词源：

antisense用法和例句：

antisense

Twenty four dogs were assigned to control group and experimental group, and experimental group was randomly divided into ACSF group, sense group and antisense group.

2 4只犬随机分为对照组和实验组 (包括人工脑脊液组、正义链组和反义链组 )。

Antisense oligonucleotide (4 ug/mL) can decrease the content of CLC-3 mRNA and make no influence on cell cycle.

ASO(4林留mL)可以减少细胞CLC一3 mRNA的含量，不

OPC colum provides a high effciency,quick and convenient method for the purification of antisense phosphorothioate Oligodeoxynucleotide to Bcl 2.

Bcl- 2硫代反义核酸 OPC纯化法是一种高效、快速、简便的方法

EMSA demonstrated that C/EBP and HMG like protein were expressed in A549 cells and A549 cells transfected with antisense NHE 1, with the expression level higher in the latter than in the former.

EMSA检测到A5 49细胞和转染NHE 1反义载体的A5 49细胞均有转录因子C EBP和HMG样蛋白的表达 ,且后者表达水平高于前者 ;

Intrathecal injection of eNOS antisense oligonucleotides inhibited the expression of NMDA1AR mRNA in the spinal cord of morphine-withdrawal rats, but did not in the brainstem.

eNOS反义寡核苷酸也可抑制吗啡戒断大鼠脊髓NMDA1AR mRNA表达的增加, 但作用弱于nNOS反义寡核苷酸, 对脑干NMDA1AR mRNA表达无明显影响。

HSP 70 antisense oligonucleotides could not only inhibit the proliferation,but also induce the apoptosis of Eca-109 cells.

HSP70反义寡核苷酸能抑制Eca-109细胞的生长并诱导其发生凋亡。

Antisense oligonucleotide with hTR

MNP-端粒酶反义核酸复合物

Results of PCR amplification and restriction digestion showed that the fragment of antisense gene CYP86MF was introduced into pBI121 plasmid and the expression vector pBI35S-AMF was transferred into Agrobacterium successfully.

PCR扩增和酶切鉴定结果表明,所构建的反义CYP86MF基因植物表达载体pBI35S-AMF是正确的,并已成功导入了根癌农杆菌中.

Both sflt-1and antisense VEGF could effectiv ely inhibited neovascularization separately,combination therapy can enhance the inhibitory effect,but t he mechanism is different.

sflt-1与反义VEGF均能有效抑制新生血管的形成,两者联合能增强抑制效果,但其作用机制不同。

The STAT3 antisense RNA modified M1 cells may be new model for studying the function of JAK STATs pathway.

STAT 3反义RNA能够在M1细胞中稳定表达 ,成为进一步研究JAK STATs途径功能的新模型。

Survivin antisense oligonucleotides could effectively induce apoptosis of drug-resistant human ovarian cancer cell line COC1/DDP.

survivin ASONs反义寡核苷酸能诱导人卵巢癌耐药细胞株COC1/DDP细胞凋亡 ,且效果显著

Apoptosis of drug-resistant human ovarian cancer cell line COC_1/DDP induced by survivin antisense oligonucleotides.

Survivin反义寡核苷酸诱导人卵巢癌耐药细胞株COC_1/DDP凋亡的实验研究

VES had no effects on p38 and ATF-2. Antisense to ATF-2 oligomers also had no effect on VES-mediated apoptotic rate.

VES 并不改变p38 蛋白的表达,且其下游转录因子ATF-2 的表达未发生改变,转染ATF-2反义寡聚核苷酸后VES 诱导的细胞凋亡亦未发生改变。

Shang ZC, Song TT, Sun BZ, et al.The influence of antisense phosphorothioate oligodeoxynucleotides on K562 celluar biology characteristic[J].Chin J Cancer, 1997, 16(4): 251-3.

[2]尚振川,宋霆婷,孙秉中,等.反义寡聚脱氧核苷酸对白血病K562细胞株生物特性的影响[J].癌症,1997,16(4):251-3.

Dai MS, Luo SK, Dai LJ, et al.Inducing apoptosis ofK562 cell lines by bcr-abl antisense oligonucleotides [J].Chin J Hematol, 1997, 5(5): 264-5.

[6]戴木水,罗绍凯,戴丽君,等.反义bcr-abl寡核苷酸诱导K562细胞凋亡的研究[J].中华血液学杂志,1997,5(5):264-5.

"This is binding in stand-by, you might say.But we can show that antisense RNA competes with the ribosomes to be able to attach to this stand-by site as well.

“你也许会说这是具有约束力的准备，但是，我们能够证实反义RNA竞争核糖体通过预备的位点。

An antisense drug, fomivirsen, is already used to treat eye infections caused by cytomegalovirus in AIDS patients.

一种叫fomivirsen的反义药物，已经用来治疗爱滋病患因巨细胞病毒所引起的眼睛感染。

Compared with A549cells,the pHi of c ells co-transfected antisense genes was remarkably decreased(P<0.05),while lactate content was remarkab ly increased(P<0.001). The cell cycle was blocked at G 0 -G 1 period.

与A549细胞比较,联合转染反义基因的细胞pHi降低、乳酸显著升高(P<0.001),细胞周期阻滞在G0-G1期。

"As compared to conventional drugs that work on proteins, antisense oligonucleotides work on RNA.

与传统的作用于蛋白质的药物相比，反义寡聚核苷酸作用于RNA。

A full-length cDNA of DFL and its antisense nucleotide sequence have been transformed into Nicotiana tabacum under the control of a cauliflower mosaic virus 35S promoter.

为了鉴定DFL基因的功能作用,构建了由35S启动DFL基因的正反义表达载体(载体中同时包含有由另外两个35S启动的潮霉素筛选基因和GUS组织化学染色基因)转化烟草。

Uppsala Uniersity scientists hae discoered an entirely new process in which short, tiny "antisense RNA" competes with the protein-producing ribosomes for starting sites for reading messenger RNA.

乌普萨那大学的科学家们发现了一个全新的进程，在短期内，微小的“反义核糖核酸”具有与制造蛋白质的核糖体竞争信使RNA起始位点的能力。

Chaikaf C,Matthews D,Appelbaun F,et al.Growth-related responses in arterial smooth muscle cells are arrested by thrombin receptor antisense sequence[J].J Biol Chem,1995,270:7431.

任国峰,王宗立,李拥军,等.反义凝血酶受体基因的构建其对主动脉血管平滑肌细胞增殖的影响[J].基础医学与临床,2000,20:47.

And there was no marked difference of MAP between ACSF group and sense group (P>0.05). The MAP of the antisense group declined significantly, but more slowly than those in the other two groups (P <0.01).

伤后3h,ACSF组与正义链组MAP之间仍无显著差异（P>0.05）,但与ACSF组和正义链组相比,反义链动物的MAP下降幅度明显减小（P<0.01）。

When the rats were repeatedly treated with antisense oligonucleotide to block synthesis of OFQ receptor, pain threshold increased significantly.

但两种剂量的0FQ阴无论是脑室还是鞘内给药，均可对抗电针镇痛；

Using specific antisense oligonucleotide as probe to selectively inhibit the gene express of receptor is a effective means for the analysis of receptor functions.

但由于其抑制效率及适用范围的不足限制了其广泛应用。

Studies in vitro as in vivo demonstrated that recombinant EGFR antisense adenovirus can effectively inhibit the proliferaion of endothelial cells and tumoral angiogenesis.

体外及体内研究均显示，EGFR反义重组腺病毒可有效地抑制血管内皮细胞的增殖和肿瘤内血管的生成。

On the other hand, antisense oligonucleotide of FAK was used to block its expression.

使用反义寡核苷酸阻断FAK的表达,达到下调信号通路的作用。

Recombinant retroviral vector expressing antisense ANG can efficiently inhibit ANG expression in A549 cell in vitro,providing the basis for further study in vivo.

利用反义血管生成素的逆转录病毒载体能够有效抑制培养细胞血管生成素的表达 ,为今后的体内应用奠定了基础。

Until now,several methods have been developed to improve the intracellular delivery of antisense oligonucleotides.

到目前为止,已发展了数种提高寡聚核苷酸类化合物细胞内转运的方法。

Including antisense oligonucleotides, ribozymes, at the wrong virus interferents, such as cell suicide gene.

包括反义核苷酸、核酶、缺陷病毒的干扰剂、细胞自杀基因等。

Antisense c-mycoligodeoxynucleotide

反义C-myc寡核苷酸

Antisense IRAK 2 ODN blocked IRAK 2 mRNA translation and protein expression was inhibited.

反义IRAK 2寡核苷酸可阻断IRAK 2mRNA的翻译 ,从而抑制IRAK 2蛋白的表达 ,抑制率达45% ;

The recombinant plasmid of antisense TIMP 1 has a certain extent reverse effects on liver fibrosis.

反义TIMP 1表达质粒对肝纤维化有一定的逆转作用

Antisense ECE was defined at the constant concentration of 1 g/100 L and 10 g/L agarose gel electrophoresis at 60 V for 1 hour.

反义内皮素转换酶核酸的浓度恒定为1g/100L,10g/L琼脂糖凝胶电泳60V电泳1h。

Antisense oligodeoxyribonueleotides (ASODN)

反义基因

Antisense oligodeoxynucleotides (ASON)

反义寡核苷酸

Antisense oligonu cleotides can specifically inhibit or drowregulate homologous gene expression, s o it might be a good choice of genetic therapy.

反义寡核苷酸可特异性抑制或下调同源基因表达，从而能成为基因治疗的首选药物。

Antisense oligonucleotides have been broadly applied in specific inhibition of gene expression in many organs, including liver.

反义寡核苷酸技术已被广泛用于在许多组织中抑制特定基因的表达。

Antisense Oligodeoxynucleotide Liposomes

反义寡核苷酸脂质体

Antisense oligonucleotide (ASON)

反义寡核苷酸（ASON）

Antisense oligonucleotides/antagonist and inhibitor

反义寡核苷酸／拮抗剂和抑制剂

Antisense oligodeoxynucleotides(ODN)

反义寡聚核苷酸

Antisense oligonucleotides have been successfully employed to inhibit and modulate specifically gene expression.

反义寡聚核苷酸已成功用于对基因表达的特异性调控与抑制。

Antisense technique is an important area of research in cancer-associated treatment.

反义技术是目前癌症治疗领域很重要的一门技术。

Antisense technology, a new method for drug design, is developed in the latest two decades.

反义技术是近20年来发展的一种全新的药物设计方法。

Antisense phosphorothioate oligodeoxynueletide

反义核酸

antisense nucleic acid technology

反义核酸技术

Antisense nucleic acid technique is a method using single-stranded complementary sequences with specificity assigned by Watson-Crick base-pairing targeted specific messenger RNA or DNA to block expression of target gene.

反义核酸技术是根据碱基互补原理，使用与目标靶的遗传物质(mRNA或DNA)特定互补的核酸片段封闭基因表达的技术方法。

As a new territory of genetic pharmacology,antisense nucleic acid drug has been promoting the development of tumor research as well as the study of genetic and infectious diseases.

反义核酸药物是基因药理学的一个新领域,对于肿瘤、遗传病以及传染病的防治具有重要意义。

Keywords C5a;Antisense peptide;Sepsis;

反义肽;脓毒症;

Antisense repetition expression vectors

反义重复表达载体

Another is to block the function of the restorer gene in a line that contains CMS cytoplasm by a way such as,RNA interference(RNAi) or antisense RNA,so that the plants would become sterile.

另一途径是利用RNA干涉或反义RNA的方法，阻断胞质不育品系（花粉可育，因为核内存在恢复基因）恢复基因（Rf）的表达，这样植物就转为花粉不育。

The needed X meanwhile produces “antisense” RNA, which acts like an antidote to protect it from Xist.

同时，另一条必要的X染色体则制造了反义RNA，以充当解药，保护自身免受Xist的作用。

Reviewed studies about moleculebiological of social recognition, it mainly based on gene-knockout mice and antisense to discuss AVP、OT and ER receptor cont...

回顾有关社会识别分子生物学方面的研究,主要是通过诸如基因敲除和反义核酸等技术来探讨升压素、催产素和雌激素受体对小鼠社会识别的功能。

The protein enelope surrounding the irus was then reersed, in a process called antisense.

在一个称作反义的过程中，病毒外部的蛋白质包膜被反转。

In this case, instead, antisense RNA sets up base pairs far away from where the reading should start?but still manages to stop the reading.

在这种情况下，反而是反义RNA从远处的碱基配对开始转录，但它仍可达成阻止转录的开始。

While the gene is producing a sensible RNA message, its alter ego can churn out an “antisense” RNA that has a complementary sequence.

基因制造出来的是同义RNA讯息，其另一半则可制造出具互补序列的反义RNA。

To achieve gene inactivation, there are four methods: gene knock out, antisense oligonuleotides, RNA interference and methylated oligonucleotides.

基因功能研究中涉及到对过度表达的基因进行抑制或者灭活。

The aim of gene therapy is to transfect DNA into target cells, in which ultilizing antisense oligonucleotides is a main method.

基因治疗是将DNA转染进入目的细胞,修复遗传错误或产生治疗因子。反义寡核苷酸的应用是基因治疗的主要手段之一。

Antisense and Nucleic Acid Drug Development

基因治疗药物开发细胞生物学

natural antisense transcripts (NATs)

天然反义转录物

Methods A recombinant antisense GFAP retrovirus was developed.After packing and concentrating, the suspension of this retrovirus was injected into the sites of injured brain stab by a microinjector.

如何抑制胶质瘢痕增生，促进神经再生与功能重建，一直是神经科学研究的重点，至今未获突破性进展。

The detailed and thorough research of these systems has provided deep insights into structure and function of prokaryotic antisense RNAs and the kinetics of antisense/sense RNA interaction.

对这些体系深入的研究揭示了原核反义RNA结构和功能的联系，以及正义／反义RNA相互作用的机理。

The hTR gene was then reverse inverted into retrovirus vector pLNCX and constructed an antisense recombinant plasmid pLNCX-aTR.

将hTR基因插入逆转录病毒载体pLNCX中，构建了hTR基因的反义重组质粒pLNCX-aTR和正义重组质粒pLNCX-TR，测序结果表明符合预期要求，为下一步实验奠定了基础。

The lignin content of transgenic triploid Chinese white poplar (Populus tomentosa Carr.) expressing the antisense 4CL (4-coumarate: CoA ligase) cDNA was remarkably reduced.

将反义4CL(4-香豆酸辅酶A连接酶,4-coumarate: CoA ligase)基因转入三倍体毛白杨(Populus tomentosa Carr.)

Meanwhile, the application of antisense gene technology is prospected.

并对反义基因技术的应用进行了展望。

Applying antisense oligonucleotide to block the expression of CLC-3 mRNA.

应用反义核酸的阻断CLC一3 mRNA在细胞中的表达。

When sense and antisense RNA molecules meet, they bind to each other and form double-stranded RNA.

当感觉和抗感觉RNA分子相遇的时候，他们结合在一起形成双链的RNA。

To generate drugs with broader capabilities than antisense RNA, my colleagues Michael Egholm, Rolf H.

当时我和同事艾格荷姆（MichaelEgholm）、柏格（RolfH.

The requirements for ideal antisense imaging include easy intakeing by cells, convenient labelling, stable hybridization and the tolerance against nuclease.

成功的反义显像要求反义寡核苷酸易被细胞摄取、耐核酸酶、杂交稳定、标记简单。

Successful acquisition of VEGFR 3 2 Ig antisense peptide would be useful in the studying the role of VEGFR 3 in cancer growth and metastasis.

成功获得VEGFR 3胞外第二免疫球蛋白样区 (2 Ig)反义多肽 ,为研究VEGFR 3在肿瘤生长和转移中的作用奠定基础。

We found that in the angiogenin antisense transfectants, the cell proliferation was decreased and the bFGF induced cell proliferation was inhibited, but the expression of bFGF was increased.

我们发现在转入反义血管生成素基因的细胞株中，细胞的增殖受到了抑制，同时bFGF诱发的细胞增殖也受到了抑制，但是bFGF的表达水平却提高了。

We transfected angiogenin gene in the sense and antisense orientation into A375 cells, and obtained stable angiogenin under-expressing and over-expressing transfectants.

我们将血管生成素的基因分别以正向和反向转入人黑色素瘤A375细胞中，得到了稳定的低表达和高表达血管生成素的细胞株。

The present study has attempted to address the above unresolved issues using antisense oligonucleotides directed against the c-myc proto-oncogene.

探讨c-myc反义寡核苷酸局部给药防治再狭窄的机制，了解c-myc反义寡核苷酸在细胞和分子水平的作用机制。

Adenovirus carrying antisense mdr1 RNA can partially reverse the MDR of HepG2/ADM cells and inhibit tumor growth by down-regulating mdr1 mRNA resulting in tumor cell apoptosis.

携带MDR1反义RNA重组腺病毒部分逆转HepG2/ADM的多药耐药,阻止肿瘤生长,下调MDR1转录水平,导致肿瘤细胞凋亡。

Abstract： Objective To study the effect of telomerase antisense RNA on telomerase activity and cell proliferation of HL-60 cells.

摘 要： 目的 探讨端粒酶反义RNA对HL-60细胞端粒酶活性及细胞增殖的影响。

The first antisense RNAs' naturally occurring were identified in plasmids and other prokaryotic accessory DNA elements.

摘要反义RNA的存在最先是在质粒和其它原核附属DNA元件中被证实的。

Objective To estimate the effort between antisense RNA probe and random primed DNA probe.

摘要目的比较不同方式标记非放射性探针的原位杂交效果。

Abstract: Survivin Antisense Oligonucleotides has the advantages of high specificity、targeting effect and non toxic and so on.It is also the Hot Research of anti-tumor and anti-virus.

摘要： 反义寡核苷酸具有高特异性、靶向性、无毒性等特有的优点，近年来一直是抗肿瘤、抗病毒领域研究的热点。

The new methods also include antisense nucleic acid technique and ribozyme technique in gene therapy...

新发展的还有反义疗法以及应用核酶进行基因治疗等。

The protein envelope surrounding the virus was then reversed, in a process called antisense.
在一个称作反义的过程中，病毒外部的蛋白质包膜被反转。