polymerase是什么意思   polymerase怎么读

polymerase单词基本解析：

polymerase变化用词：

polymerase英英释义：

1. an enzyme that catalyzes the formation of new DNA and RNA from an existing strand of DNA or RNA

名词 polymerase:

an enzyme that catalyzes the formation of new DNA and RNA from an existing strand of DNA or RNApolymerase[ 'pɔlimə,reis, -reiz ]n.an enzyme that catalyzes the formation of new DNA and RNA from an existing strand of DNA or RNA

polymerase中文词源：

polymerase用法和例句：

Methods Polymerase chain reaction ( PCR ) connected with reverse dot blot ( RDB ) were performed.

方法采用多聚酶链反应 ( PCR ) 结合反向斑点杂交 ( RDB ) 技术.

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Methods Reverse transcription - polymerase chain reaction ( RT - PCR ), and immunohistochemical method were used.

方法采用反转录 - 聚合酶链反应 ( RT -PCR ) 法和免疫组织化学方法.

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DNA molecule to RNA polymerase binds, initiating the transcription of RNA.

一种rna模板上的dna形成的聚合酶, 尤其在逆转录酶病毒中发现.

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Inhibits platelet aggregation. DNA polymerase inhibitor. Shows antifungal activity.

抑制血小板聚集. DNA聚合酶抑制剂. 显示抗真菌活性.

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The sex of embryos were determined with PCR ( Polymerase chain reaction ) technique.

胚胎的性别鉴定采用多聚酶链式反应 ( PCR ) 技术.

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Researchers believe that the bird virus polymerase allows for faster replication, and thus more aggressive.

研究人员认为,禽流感病毒聚合酶允许更快的复制, 从而更加积极.

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Objective To develop a novel multiplex polymerase chain reaction ( PCR ) to detect multidrug - resistant Acinetobacter baumannii.

目的建立一种适于临床微生物实验室快速检测多耐药 鲍曼 不动杆菌基因的多重PCR方法.

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TLR 4 mRNA in KCs was determined by the reverse transcription polymerase chain reaction ( RT - PCR )

用逆转录多聚酶联反应 ( RT -PCR ) 测定KCs中TLR4mRNA的表达.

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The expression of TLR 4, MD - 2 mRNA were assayed by reverse transcriptase polymerase chain reaction ( RT - PCR ).

逆转录聚合酶链反应 ( RT -PCR ) 检测各组细胞TLR4和MD-2mRNA的表达.

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Objective To establish DNA typing for HLA - DR antigens polymerase chain reaction with sequence - specific primers ( PCR - SSP ).

目的采用顺序特异引物聚合酶链反应 ( PCR -SSP ) 建立人类白细胞抗原DR位点的DNA分型方法.

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Objective To discuss the feasibility and superiority of diagnosis of acanthamoeba Keratitis using polymerase chain reaction.

目的探讨聚合酶链反应(PCR)技术临床应用于棘阿米巴角膜炎诊断的可行性及优越性.

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The gene expression of ET - 1、 ETAR 、 ETBR and ECE was evaluated by semi - quantitative reverse transcription polymerase chain response ( RT - PCR ).

采用半定量逆转录多聚酶链反应 ( RT -PCR ) 检测局部内皮素系统ET -1 、ETAR、ETBR及ECE的基因表达.

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Objective To develop a quantitative real - time polymerase chain reaction ( PCR ) for detecting Bartonella henselae.

目的采用新型TaqMan -MGB 探针建立检测汉赛巴通体的实时荧光定量PCR方法.

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Semi - quantitative reverse transcription - polymerase chain reaction ( RT - PCR ) was used to detect the level of MMP - 12 mRNA expression.

以 半定量 RT -PCR 法检测MMP -12 mRNA表达水平.

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It was validated the extracted DNA could meet the need of polymerase chain reaction ( PCR ).

涂渍的芯片所提取出的DNA能够满足聚合 酶 链反应的需要.

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polymerase

For his invention of the polymerase chain reaction (PCR) method.

(聚合酶链反应法的发明)。

J. 1993.Detection of herpesvirus infection by polymerase chain reaction.

1993.以聚合脢连锁反应检测假性狂犬病毒核酸。

The genome of NS5A and HVR1 region were amplified by the methods of Reverse Transcription Polymerase Chain Reaction ( RT - PCR).

HCV HVR1及NS5A基因片断扩增：采用巢式逆转录聚合酶链反应法(RT-PCR)。

Acquisition of CagA and CTB genes:By in situ polymerase chain reaction (PCR) method, CagA was amplified from Hp chromosomal DNA and CTB from Vibrio cholerae.

Hp CagA和CTB基因的获取

The KOH preparation was consistent with a fungal pathogen, which was later identified as Blastomyces dermatitidis by polymerase chain reaction.

KOH标本与真菌病原体一致，经聚合酶链反应确定为皮炎芽生菌。

Activity of poly (ADP ribose) polymerase (PARP) was determined by NAD incorporating method.

NAD参入法测定了这些细胞的聚（腺苷二磷酸?核糖）聚合酶（PARP）活性。

PCR, or polymerase chain reaction, can build copies of even the tiniest fragments of deteriorated DNA to allow accurate DNA typing.

PCR也就是聚合酶连锁反映，能制成即便是变质的dna最微小的碎片的复制品，从而使dna分类达到精确。

PCR stands for Polymerase Chain Reaction and is a common way of testing for a variety of different organisms.

PCR就是聚合酶链式反应的缩写，是检测多种不同生物的常用技术。

How far along the DNA would the transcription "bubble" formed by RNA polymerase move in 10s?

RNA聚合酶形成的转录泡在10秒内可以移动多远?

The RNA polymerase unzips a small portion of the DNA helix exposing the bases on each strand.

RNA聚合酶解开一小段DNA螺旋，使碱基在它们各自的链上暴露出来。

The difference of bone morphogenic protein 2 (BMP 2) mRNA expression in hMSC and osteoblasts was compared by reverse transcriptase polymerase chain reaction(RT PCR).

RT-PCR法比较骨形成蛋白(BMP)-2mRNA在hMSC和成骨细胞中的表达差异。

SSR(simple sequence repeat),a kind of DNA molecular genetic maker based on PCR(polymerase chain reaction),existed extensively in plant genome.

SSR(simple sequence repeat)是一种基于PCR(polymerase chain reaction)的DNA分子遗传标记,广泛分布于整个植物基因组。

This led us to suppose that the mutated RNA polymerase was incapable of transcribing certain sporulation genes and so blocked spore formation.

上述结果说明利福霉素能以某种方式影响RNA聚合酶的构象或它的修饰，从而导致孢子形成性状的改变。

Comparative Study of Six Kits of Polymerase Chain Reaction Assay with Cell Culture and Ligase Chain Reaction Assay in Detection of C.

与<<细胞培养、连接酶链反应和六种聚合酶链反应试剂盒检测沙眼衣原体的比较研究>>相似的文献。

Consistently, we showed that Dimethylsulfoxide dramatically elevated the RNA polymerase activity and slightly increased the affinity (K_M) with the DNA template.

与之相一致的是,我们的数据显示了二甲亚砜极为显著的提高了RNA聚合酶的活性,同时,并不改变RNA聚合酶对DNA模板的亲和性(K_M值)。

The denatured DNA is a suitable template for transcription of the two RNA polymerase B.

两种RNA聚合酶B都适合于利用变性的单链DNA作转录模板。

In the Egloff et al. study, mutation of serine-7 to alanine does not block recruitment of polymerase II to genes, but blocks the processing of nascent RNA transcripts.

乘以磷酸化的重复又怎么样？

What other people think will be the polymerase which are small molecules in the fusion replications of viruses by setting polymerase.

也有人认为是聚合酶，他们是通过释放聚合酶影响病毒融合复制过程的小分子蛋白酶。

Long-term treatment with the second-generation nucleoside analogue lamivudine (lam) efficiently inhibits HBV replication with frequent viral polymerase mutations.

二代拉米夫定的长期应用可以有效地抑制病毒多聚酶突变频繁的HBV发生复制。

The fingerprints amplification by polymerase chain reaction(PCR) with 6 polymorphic 10-based random primers selected from 40 ones was analyzed.

从40个引物(10碱基的寡核苷酸)中筛选出6个引物,对它们的PCR扩增结果进行分析。

The stress-inducible transcription factor DREB1A gene is amplified from Arabidopsis thaliana (Columbia ecotype) genome by polymerase chain reaction and cloned into pUC19 vector.

以拟南芥基因组DNA为模板，通过PCR扩增得到逆境胁迫诱导转录因子DREB1A基因，将其克隆到pUC19质粒中。

Our result showed allele-specific polymerase chain reaction can rapidly differentiate the infection caused by M leprae or by M tuberculosis.

使用对偶基因特异性聚合连锁反应可以快速正确鑑别诊断麻风及结核感染。

The plasmids of pFUW80 and pSSK-I were used as templates for polymerase chain reaction (PCR).

分别以质粒pFUW8o一6XVHCDR3、pssK一I为模板，进行聚合酶链反应(pCR)。

The expressions of Slit2 were tested by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical measurement.

分别通过逆转录聚合酶链反应法(RT-PCR)和免疫组织化学方法检测Slit2基因的表达水平及部位。

Beta2-toxin gene was amplified from chromosomal DNA of Clostridium perfringens type C by polymerase chain reaction(PCR).

利用PCR技术,从C型产气荚膜梭菌染色体基因组中扩增了0。

Using a reverse transcriptase polymerase chain reaction (RT-PCR) the 540bp-long S1 gene of two strains of avian Reovirus (ARV) (S1133,1733) were amplified successfully.

利用RT-PCR(Reverse Transcriptase-Polymerase chain Reaction)技术,扩增出禽呼肠孤病毒S1133、1733两毒株长为540bp的S1基因片段。

A cDNA clone encoding a pheromone binding protein 3 from antenna of Helicoverpa assulta (named HassPBP3) was isolated by reverse transcription polymerase chain reaction (RT-PCR).

利用RT-PCR技术从烟实夜蛾Helicoverpa assulta( Hass)雄虫触角中扩增得到了信息素结合蛋白3( HassPBP3)。

Expression of apoptosis-related proteins(Caspase-3, Caspase-8 and poly ADP-ribose polymerase(PARP)) was determined by immunohistochemistry.

利用间接免疫荧光技术和免疫细胞化学技术检测细胞内凋亡相关蛋白Caspase-8、Caspase-3以及死亡底物聚ADP核糖聚合酶[poly(ADP-ribose)polymerase,PARP]的表达活性变化。

Even minute amounts of DNA can now be amplified, using the POLYMERASE CHAIN REACTION, to provide sufficient material for genetic fingerprinting.

即使仅获得很少量的DNA也可使用聚合酶链式反应来扩增以提供足够的材料进行遗传指纹分析。

Arnheim N, Erlich H. Polymerase chain reaction strategy. Annu Rev Biochem, 1992, 252:1643.

叶顺章，聚合酶链反应在性病诊断中的应用，中华皮肤科杂志，1996，29/p

Later that L19RNA under certain conditions, can catalyze specific oligonucleotide at the end of the cutting and connecting with and RNA polymerase activity.

后来发现四膜虫L19RNA在一定条件下能专一地催化寡聚核苷酸底物的切割与连接，具有核糖核酸酶和RNA聚合酶的活性。

A DNA molecule to which RNA polymerase binds, initiating the transcription of messenger RNA.

启动区和核糖核酸聚合酶相结合的脱氧糖核酸分子，它引起核糖核酸信使的转录

A DNA molecule to which RNA polymerase binds,initiating the transcription of messenger RNA.

启动区和核糖核酸聚合酶相结合的脱氧糖核酸分子，它引起核糖核酸信使的转录。

Further studies showed that DNA replication fidelity is decreased and there is an alteration of DNA polymerase spectrum when mammalian cells are attacked by MNNG.

因此，我们推测，哺乳细胞中低保真度的跨损伤DNA聚合酶可能参与了MNNG诱导的哺乳细胞非定标突变。

In this work, we first evaluate a polymerase chain reaction (PCR) based method for diagnosis of leptospirosis.

在本文中，我们首先评估了通过PCR法检测钩端螺旋体病的方法。

Pig GATA-3 cDNA was obtained by reverse transcription polymerase chain reaction (RT-PCR), using in silico cloning strategy based on pig dbEST.

基于猪的表达标签数据库电子克隆了猪的GATA-3基因，并通过RT-PCR验证了猪的GATA-3基因的核苷酸序列。

Beta-exotoxin is an inhibitor of RNA polymerase and acts competitively with ATP in various biological processes.

外毒素是RNA聚合酶的抑制剂，在各个生化过程中与ATP起竞争作用。

Quake's systems can sort cells, perform the polymerase chain reaction to amplify DNA, or grow protein crystals.

奎克的系统可以做细胞分类、进行聚合酶连锁反应以放大DNA，也可以培养蛋白质晶体。

One of its jobs is to pry apart the two strands of DNA (via helicase action) to allow the RNA polymerase to get access to the DNA bases.

它的其中一个工作，是探查两条分开的DNA链（通过螺旋运动方式），以使RNA聚合酶可以与DNA碱基相接触。

These results suggested that T7 promoter functions as cis-acting elements of RNA polymerase transcriptional system in eucaryotic cells.

实验结果表明T7启动子可作为RNA聚合酶的顺式作用因子。

A 40-base polymorphic repeat sequence located in the 3'-untranslated region of the DAT gene was purified and amplified by polymerase chain reaction (PCR).

将位于多巴胺运输器基因3'端未转译区段的40-碱基多形性重复序列予以纯化、经聚合酶链锁反应放大。

A 40base polymorphic repeat sequence located in the 3'untranslated region of the DAT gene was purified and amplified by polymerase chain reaction (PCR).

将位于多巴胺运输器基因3'端未转译区段的40碱基多形性重复序列予以纯化、经聚合酶链锁反应放大。

Interfering with polymerase function could proide new ways to treat or preent flu," says Cusack, "but this will require a detailed picture of the rest of the polymerase.

库萨克说’扰乱多聚酶的功能能够提供治疗或阻止流感的新方法,但是这将需要剩余的多聚酶的详细图片.

The brain samples were detected by reverse transcription polymerase chain reaction(RT-PCR).Then the PCR-positive samples were injected mice cerebrally.

应用RT-PCR技术对犬脑、蝙蝠和野鼠脑组织进行狂犬病病毒检测,并将阳性材料进行小白鼠脑内接种试验。

The histochemical staining and polymerase chain reaction was used to detect oral leukoplakia.

应用组织化学染色法和聚合酶链反应技术对口腔白斑是否存在念珠菌进行检测。

The method of polymerase chain reaction(PCR)was used to detect the specific DNA of mycoplasma pneumoniae(MP)in 140 children.

应用聚合酶链反应(PCR)枝术对140例小儿咽拭子标本进行了肺炎支原体(MP)特异性DNA检测。

Detection deleted Duchenne muscular dystrophy carriers by fluorescence quantitative polymerase chain reaction.

应用荧光定量PCR检测缺失型DMD携带者的研究

A method of one-step reverse transcription polymerase chain reaction was developed to subtype hemagglutinin (HA) gene of Avian influenza virus (AIV).

建立一步法RT-PCR检测方法，对禽流感病毒(Avian influenza virus, AIV)的血凝素(Hemagglutinin, HA)分型进行了研究。

PCR (polymerase chain reaction) is a very sensitive test that is used when there are no CML cells found by FISH .

当FISH检测不出白血病细胞时，可以采用PCR（聚合酶链反应）法，它的灵敏度极高。

As bases are added by polymerase to the starting point of a new complementary strand, known as a primer, or recognized by ligase as a match, the template's sequence is revealed.

当聚合酶将一个核苷酸加在新互补链的起始引子之后，或接合酶认定某段核苷酸链与原始模版配对，就可利用这些反应来得出原始模版的序列。

We amplified SSU rRNA of Leishmania donovani with primer R222 and R333 by reverse transcription polymerase chain reaction.

我们采用引物R222和R333建立杜氏利什曼原虫SSUrRNA逆转录多聚酶链反应（RT－PCR）。

Ramachandran Plot suggested that the three-dimentional model of FMDV strain AF72 3D polymerase was rational.

拉马钱德兰图证明，构建的3D聚合酶的空间结构是合理的。

Objective] To investigate nucleotide polymorphisms of five exons in human poly(ADP ribose)polymerase 1(PARP 1) gene.

探讨人类聚腺苷二磷酸核糖聚合酶-1(PARP-1)5个外显子核苷酸多态性。

To investigate the application of the polymerase chain reaction (PCR) in diagnosis of oral candidal leukoplakia.

探讨聚合酶链反应技术在检测口腔白斑中念珠菌感染的应用。

The RNA polymerase activity of H37Rv strain was strongly inhibited by RFD, but not that of the resistant strain.

提取及分析结核杆菌RNA聚合酶,发现敏感菌酶活性受利福定明显抑制,耐药菌酶活性则未见抑制。

Total RNA was isolated and gelatinase A (MMP2) gene expression level were rneasured by reverse transcription polymerase chain reaction (RT-PCR).

提取组织总RNA；用逆转录共扩增定量PCR方法测定明胶酶A（MMP2）基因表达水平。

ASTRACT Aim Detection of chlamydial infectious condition in newborn with polymerase chain reaction.

摘 要 目的 用聚合酶链反应检测新生儿衣原体感染状况。

Adult hen (white leghorn) was immunized with 615 mouse liver RNA polymerase B.

摘要 用615小鼠肝RNA聚合酶B免疫母鸡获得了抗血清。

The telomerase activity in mouse oocyte and preimplantation embryo was examined using the polymerase chain reaction-based on telomeric repeat amplification protocol (TRAP).

摘要为了解小鼠早期发育过程中端粒酶的活性变化，本研究利用端粒重复序列扩增法(TRAP)进行了小鼠卵母细胞和附植前胚胎端粒酶活性的测定。

To study inborn duck hepatitis B virus model in Guilin area and find out the possibility of using polymerase chain reaction (PCR) method in DHBV detection.

摘要目的了解桂林地区幼龄麻鸭携带鸭乙型肝炎病毒(DHBV)的情况并探讨PCR用于DHBV检测的可能性。

The polymorphism of DGAT1's 3'UTR in Chinese Holstein was detected through PCR-SSCP (Polymerase Chain Reaction-Single Strand Conformation Polymorphism).

摘要采用PCR-SSCP分子标记方法对中国荷斯坦奶牛DGAT1基因3'非翻译区进行了多态性分析。

Total RNA of islets were abstracted by TRIzol reagent. The levels of bcl-2 and bax mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR).

收集各组细胞，采用TRIzol试剂RNA提取法分别提取各组细胞总RNA，逆转录多聚酶链反应(RT-PCR)检测Bcl-2及Bax mRNA表达情况。

Method:The HEp 2 cells after elemene treatment were analyzed utilizing Westernblot and reverse transcriptase polymerase chain reaction (RT PCR).

方法 :使用比色法 ,研究榄香烯诱导人喉鳞状细胞癌细胞株HEp 2细胞凋亡时Caspase 3活性的变化 ;

Methods VDR( Bsm I)gene polymorphisms of 186 healthy pre-menopausal women were detected with polymerase chain reation-restriction fragment length polymorphism (PCR-RFLP).

方法 :应用 PCR- RFL P技术检测 186例绝经前健康妇女的 VDR基因Bsm I的多态性。

Method:By using reverse transcriptase polymerase chain reaction (RT PCR), S, mdr 1 and LRP gene expression were studied in 46 de novo AML cases.

方法 :应用RT PCR法检测 4 6例初治AML患者的S、mdr 1和LRPmRNA表达的阳性率。

Methods: TTV DNA was detected in serum and PBMC of 66 hemodialysis patients by polymerase chain reaction (PCR). HBsAg and Anti HCV in sera was detected by EIA.

方法 :采用聚合酶链反应 (PCR)对 6 6例血液透析患者血清及PBMC进行TTV DNA检测 ,同时采用酶免疫分析 (EIA)检测血清中HBsAg和抗 HCV。

Methods: Mainly with methods of in situ reverse transcription polymerase chain reaction (In situ RT-PCR).

方法 以原位逆转录聚合酶链式反应(In situ reverse transcription polymerase chain reaction,In situ RT-PCR)为主要研究方法。

METHODS Using reverse transcription polymerase chain reaction (RT PCR),the expression of CaM mRNA was measured in HPAA of rats which were injected with acetic acid cortisone.

方法 以逆转录多聚酶链反应 (RT PCR)半定量法测定大鼠注射醋酸可的松后钙调素 (calmodulin ,CaM)mRNA的表达水平。

Methods Genomic DNA was isolated, and cytochrome c oxidase I (COI) and cytochrome b (Cyt b ) genes were amplified by polymerase chain reaction (PCR).

方法 实验室饲养的F1钉螺 ,单个抽提基因组DNA ,PCR扩增细胞色素c氧化酶I(COI)、细胞色素b(Cytb)基因并测序 ,用GENETYX MACver .

Methods Deletion analysis of the probands was performed by multiplex polymerase chain reaction (PCR) to amplify 9 dystrophin exons described by Chamberlain.

方法 用多重聚合酶链反应方法扩增dystrophin基因 9个外显子 ,检测先证者有无外显子缺失 ;

Methods The bfp A and eae A gene of EPEC were detected by polymerase chain reaction directly from feces and the strain was isolated by tradition method.

方法 用聚合酶链反应直接从粪便标本中和传统方法分离后获得的菌株中测定bfpA和eaeA基因。

Methods The expressions of FN gene in 12 cases of gastric malignant lymphoma were detected by gene CMOS chip and reverse-transcriptase polymerase chain reaction-(RT-PCR).

方法 运用基因芯片和逆转录聚合酶链反应 (RT -PCR)技术检测 12例胃恶性淋巴瘤细胞中FN基因表达。

Methods The (CGG) n in the FMR-1 gene was amplified by polymerase chain reation (PCR) and then detected by the hybridization with biotin-labeled CGG oligonucleutide.

方法 采用生物素标记的CGG寡核苷酸探针 ,检测通过PCR扩增的FMR - 1基因中CGG三核苷酸重复序列数目。

Methods :P53 gene mutation patterns in 20 cases of HCC by polymerase chain reaction (PCR) and PCR prod-ucts sequencing.

方法:应用多聚酶链式反应(PCR)结合PCR产物测序,同时检测肝癌P53蛋白表达。

Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of PTEN and MMP-7 mRNA in 46 cases with gastric cancer.

方法:应用逆转录-聚合酶链反应(RT-PCR)方法,对15例癌旁组织和46例胃癌组织中PTEN和MMP-7基因mRNA的表达进行分析。

Methods Candida albicans was geno- typed using RAPD under the different conditions of primer,template,concentration of Mg~(2+)and dosage of Taq polymerase.

方法:选用不同的引物、Mg~(2+)、模板浓度、Taq聚合酶用量等,获得一优化的反应体系以对白假丝酵母菌进行RAPD分型。

Method Polymerase chain reaction(PCR) based-on LOH was performed in 42 primary ESCC with 5 microsatellite markers.

方法:采用PCR银染技术,检测42例配对食管鳞癌标本D5S346和D5S82的杂合性丢失情况。

Methods:The methods of polymerase chain reaction-sequence specific primers(PCR-SSP) was used to genotype ABO blood group.

方法:采用聚合酶链反应-序列特异性引物(PCR-SSP)基因定型方法对ABO疑难血型进行检测。

METHODS: Twenty-three fresh resection samples of colorectal carcinomas were studied by the polymerase chain reaction (PCR) to detect the loss of heterozygosity of DCC.

方法:采用聚合酶链反应方法(polymerasechainreaction,PCR)对23例结直肠癌组织新鲜标本DCC基因杂合性缺失(lossofheterozygosity,LOH)进行研究。

Methods The expression of ANP and NPR-A in the rat cochlea of different age groups were examined by RT-PCR(Reverse transcriptase polymerase chain reaction).

方法分离不同年龄段大鼠耳蜗组织,采用RT-PCR方法检测ANP及其受体NPR-A的表达。

The histochemical staining and polymerase chain reaction was used to detect oral leukoplakia.
应用组织化学染色法和聚合酶链反应技术对口腔白斑是否存在念珠菌进行检测。