plasmid是什么意思 plasmid怎么读
英式：['plæzmɪd]
美式：['plæzmɪd]
plasmid单词基本解析：
n.质粒，质体n.[生]质粒,质体
n.质粒，质体；
plasmid变化用词：
plasmid英英释义：
Noun1. a small cellular inclusion consisting of a ring of DNA that is not in a chromosome but is capable of autonomous replication
名词 plasmid:
a small cellular inclusion consisting of a ring of DNA that is not in a chromosome but is capable of autonomous replication
plasmid[ 'plæzmid ]n.a small cellular inclusion consisting of a ring of DNA that is not in a chromosome but is capable of autonomous replication
plasmid中文词源：
plasmid用法和例句：
Objective To construct the plasmid expressing BDV p 40 gene.
目的构建博尔纳病病毒p40基因重组表达质粒.
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Objective : To find a rapid method of screening positive recombinant plasmid.
目的: 建立一种快速筛选阳性重组质粒的方法.
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Conclusions The recombinant plasmid constructed is useful for establishing a transgenic mouse.
构建所得的重组质粒可用于下游的转基因工作.
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The recombinant CYP 3 A 4 plasmid was successfully cloned and effectively expressed in Rosetta ( DE 3 ) 2 pLysS.
成功地使CYP3A4基因在原核系统中高效表达.
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Objective : To survey plasmid profiles ( PP ) of Neisseria gonorrhoeae in Nanchang.
目的: 研究南昌地区 淋病 奈瑟菌质粒 谱 型的分布情况.
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BVTech Plasmid is DNA plasmid drawing software free software.
画质粒图软件,可绘出用于发表文章的基因图.
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Lysosome and cellular plasmid are activated to restrain activity of heavy metals.
溶酶体和细胞质粒抑制重金属活性.
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Objective Construct a recombinant plasmid pET 28 a - EDA - EDB , prepare the fusion EDA - EDB protein.
目的构建重组pET28a-EDA-EDB 质粒, 制备重组纤维连接蛋白EDA -EDB 融合蛋白.
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Figure 2 . Restriction pattern of the recombinant plasmid.
图2. 阳性重组子质粒酶切电泳图谱.
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The PNL - BDNF - IRES 2 - EGFP plasmid was identified to be correct.
构建的慢病毒载体质粒PNL -BDNF-IRES2-EGFP 经SalI和 BamHI 双酶切鉴定正确,三质粒共转染293T细胞 后荧光激发可见大量绿色荧光.
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Results: The recombinant plasmid pUC 18 E 6 had been got.
结果: 获得重组质粒pUC18E6.
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PC - 3 cells were transfected with mGM - CSF expressing plasmid or the control vector by lipo - some transfection.
通过脂质体转染法,将含GM -CSF 基因的质粒及空质粒转染至PC -3 细胞.
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Objective To explore the elimination effect of radix Pulsatillae extracts on R plasmid of pseudomonas aeruginosa.
目的探讨中药白头翁提取液对铜绿假单胞菌R质粒的消除作用.
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Objective To evaluate the therapeutic effect of VEGF plasmid in cerebral infarction of dog by MR - DWI.
目的 用MR -DWI 追踪VEGF质粒对家犬脑梗塞模型的治疗效果.
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Replication, transcription and origin of the two kinds of mitochondrial plasmid are introduced respectively.
还分别介绍了它们的复制机制 、 转录和起源.
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plasmid
ALR gene was also inserted to bicistronic plasmid p! RES2-EGFP to produce ALR, EGFP gene bicistronic eukaryotic expression vector (pIRES-EGFP/ALR).
ALR和EGFP基因双顺反子表达载体，pIRES-EGFP/ALR。
ALR gene was inserted into pEGFP-Cl plasmid to construct ALR and EGFP co-expression vector pEGFP/ALR.
ALR和EGFP融合基因表达载体，pEGFP/ALR。
Furthermore,the plasmid pRY3 was constructed,in which the nanA gene is controlled by the tac promoter .
coliBL21(DE3)/pRY1 中,nanA 基因受lac启动子控制,为IPTG 或乳糖所诱导表达。
Recombinant plasmid, pBYBl, with full length cry1Aa14 gene was constructed and transformed into a Bt acrystalliferous mutant strain HD73 cry.
cry1Aa14全长基因插入Bt表达载体，获得了重组表达质粒pBYB1，转化大肠杆菌SCS110和Bt无晶体突变株HD73cry~-，经过抗性筛选、DNA酶切分析和PCR检测，证实转化成功。
Then we have cloned the mouse DOC - 1R cDNA sequence into the pMEISS - FL3 plasmid.
pcDNA3-DOC-1R反义重组质粒的构建
ICAM-2 promoter fragment and DAF-intron 1 fragment were produced by using PCR technique. These fragments and DAF cDNA were cloned into pGEM-7Zf plasmid and sequenced.
PCR插入法将人 ICAM-2启动子、人 DAF-intron1和 DAFc DNA克隆到 p GEM-7zf质粒中 ,进行序列测定及分析 .
It was observed to transpose from plasmid pFD13 to plsmid R144drd3, or to the E. colt chromosome.
Tn2981可以从质粒pFD13转座到质粒R144drd3上,也能从质粒转座到大肠杆菌的染色体上。
TNA may enhance transfection rate of plasmid DNA mediated with liposome, and may be beneficial to the treatment of cancer.
TNA可促进脂质体介导质粒DNA转染结直肠癌细胞 ,将营养支持与基因治疗相结合 ,有望提高对肿瘤患者支持治疗效果。
The molecular biological study of the mechanism of drug resistance at gene and plasmid level have made a great progress.
“在耐药机理研究上，分子生物学水平的基因和质粒研究进展很大”。原译文为:
The preservation subjects include microbiology, cell line, plasmid et.al.
“病原库”对外服务的业务范围包括：微生物的分离、鉴定、保藏；
Full length NS3 gene of a hepatitis C virus was amplified by PCR using plasmid pBAC25 containing HCV nonstructural protein gene as template.
丙型肝炎病毒(HCV)是引起非甲非乙型肝炎的主要病原因子。
Study on Expression of Recombinant Plasmid PsecTaq2A-AMG for Human Amelogenin in Human Gingival Fibroblast.
人釉原蛋白重组质粒Psec Taq2A-AMG在人牙龈成纤维细胞表达的研究
Two RAPD fragments of Siniperca chuatsi virus (SCV) genome DNA recovered from agrose gel were inserted into plasmid pUC19 (called SCVE369 and SCVE450).
从RAPD扩增的鳜鱼病毒 (SCV)核酸电泳带中回收了二个片断 ,克隆子pUC19质粒 (称为SCVE36 9和SCVE45 0 )。
The plasmid pJW566 was isolated from L.lactis subsp. cremoris W56,one strain for Danish chadder mixed starter cultures.
从丹麦乳酪发酵启子乳酸乳球菌乳脂亚种 (Lactococcuslactissubsp .cremoris)W56中 ,分离到一个 2 2 4kb的质粒pJW566,将该质粒转化到无质粒且噬菌体敏感的L .
Amplified of the green fluent protein (GFP) gene from plasmid PCR3.1, and add suitable restriction enzyme cutting site.
从含有gfp基因的质粒pCR3.1上扩增出全长基因，并且加上合适的酶切位点。
The recombinant plasmid pPGVT3 derived from the vector pUC4 was unstable in the E.
从载体质粒pUC4衍生的重组质粒pPGVT3在大肠杆菌宿主DF2145中是不稳定的,以pPGVT3转化DF2145时在4o℃培养得不到转化子。
The different of the two plasmid lies in different Promotors,since a strong promotor may paly a important role in gene expression in viral vector.
他们的区别在于含有不同的启动子，因为强启动子可能在病毒载体系统的基因表达上起关键的作用。
A doublegene plant expression vector,pC35SC35SB1303 were reconstructed based on PC1303 plasmid by CMO and BADH which were both driven by 35S promoter.
以pC1303质粒为基础,构建了均由35S启动子驱动的CMO基因和BADH基因的植物双基因表达载体pC35SC35SB1303。
The green fluorescent protein-C2 (GFP-C2) plasmid was used as target gene. SDION-GFP-C2 compounds were synthesized by oxidation-reduction reaction.
以绿色荧光蛋白(GFP-C2)质粒为靶基因,通过氧化还原法构建SDION-GFP-C2复合物,用紫外分光光度计和琼脂糖凝胶电泳检测两者的结合率。
Then the recombinant plasmid was transfected into the COS7 cell line and identified its expressing of protein by Western blot analysis.
以脂质体法将pcDNA3.1(+)-flk1-domainl-7转染COS7细胞,用Western blot方法对目的基因蛋白质的表达进行鉴定。
The parasporal body will be expressed in Bacillus thuringiensis only if a specialized plasmid is present.
伴胞体只有在特定质粒存在的条件下才会在苏云金芽孢杆菌中表达。
The expression of the plasmid in vivo has a chemotactic effect on immune cells and can inhibit the growth of solid tumor.
体内表达可对免疫细胞产生趋化作用，并可抑制实体瘤生长。
Assuming that the plasmid DNA is endotoxin-free, mention how you think "danger" is signaled by DNA of bacterial or plasmid origin (5 points).
假设这个质体DNA不含内毒素，提出理由说明你认为"危险"讯息是源自细菌的DNA或是质体（5分）。
He described the technique to put this gene inside plasmid cationic liposome complexes and then deliver it intravenously.
先将环氧合酶基因导入质粒阳离子脂质体复合物，再通过静脉给入。
Title： Construction and Identification of Recombinant Plasmid of TUB2 Gene and Transformation into Chaetomium sp.
关键词：抗苯并咪唑类基因,质粒,构建,鉴定,转化
Title： Study on transfering and diffusing of antibiotic resistant plasmid in Shigella spp.
关键词：痢疾杆菌；耐药性；转移；扩散
The co expression plasmid encoding FasL and VEGF165 named pCI FasL IRES VEGF165 (simplified as pCI FIV) was successfully constructed.
再以此为基础构建得到编码FasL和VEGF165的共表达质粒 pCI FasL IRES VEGF165 (简称pCI FIV )。
We have fused lac structure gene into mer of plasmid R100.1 by means of Mudl (Apr lac) phage, and got 47 strains of mer-lac gene fusion.
利用Mud1(Ap~rlac)噬菌体将lac结构基因融合到质粒R100.1的汞基因(mer)中,得到了47株mer-lac基因融合菌株。
The damage effect of plasmid DNA and mutation induced of lacZ gene were analysesed by low power HeNe laser radiation invitro plasmid DNA.
利用低功率HeNe激光辐照离体质粒DNA，分析了质粒DNA的损伤效应和lacZ基因的突变。
A recombinant plasmid pUC-IL6 coding for IL6 was constructed by recombinant gene technique.
利用基因重组技术，构建含IL6基因的重组质粒pUC－IL6。
The recombinant plasmid was packaged and amplified after being transfected in HEK293 cells through Lipofectamine.
利用脂质体介导重组腺病毒载体转染HEK293细胞，包装出完整的腺病毒；
Function of procayon and plasmid: to carry and pass on genetic information.
原核和质粒的功能：携带和传递遗传信息。
In addition to encode many special characters,plasmid of bifidobacteria also can be used as tools to express various exogenous genes.
双歧杆菌质粒除了编码许多特殊性状外,还是在双歧杆菌中表达各种外源基因的重要工具。
The recombinant plasmid of antisense TIMP 1 has a certain extent reverse effects on liver fibrosis.
反义TIMP 1表达质粒对肝纤维化有一定的逆转作用
In addition, the amplified IL-15 cDNA fragment from plasmid pBS-IL15 was digested with BamH I and cloned into EcoR I (filled in)/BamH I pBV220 vector.
另外，将经BamHI酶切的IL-15cDNA扩增片断与经EcoRI酶切、补平、再经BamHI酶切的pBV220载体相连，构建成重组质粒pBVB-IL15。
In addition, a set of expression plasmid vectors, PMS-31b.
同时构建一组质粒表达载体PMS-31b。
The enhanced green fluorescent protein(EGFP) plasmid was used as positive control and the transfection efficiency was detected by flow cytometry.
同时转染带有增强型绿色荧光蛋白(EGFP)的载体作为阳性对照,流式细胞术检测其绿色荧光以确定转染效率;
Four strains of K. pneu were chosen to make transfer experiments for confirming the presence of transferable plasmid ampC genes.
同时选择4株菌(3株ampC基因阳性,1株阴性)进行转质粒试验,以证实质粒型基因的存在。
A bacteriophage, a plasmid, or another agent that transfers genetic material from one location to another.
噬菌体、质体或其它介体把遗传物质从一个地方转到另一地方。
CoNS were typed with plasmid and PFGE(pulsed field gel electrophoresis) profiles.
四川大学华西第二医院;
The research of pCKM-mPTH recombinant plasmid therapy for hypoparathyroidism has been deployed, and it is only reported by us in the whole world.
国际上率先开展pCKM-mPTH重组基因体系治疗甲状旁腺功能低下症的实验研究。
Fig.5 The Linear and superhelix DNA of PBS plasmid,L:linear DNA,SU:superhelix DNA.
图5PBS质粒的线状及超螺旋DNA结构，SU：为DNA的超螺旋结构，L：为DNA的线状结构。
And plasmid profiles were clear respectively on agarose gel (0.5%), which were named P1,P2, P3 and P4 by their molecular weights.
在0.5%琼脂糖凝胶电泳中，质粒DNA分离相当清晰。 Bt 4.0718菌株含有4个大小不等的质粒，依据其分子量大小顺序依次编号为P_1、P_2、P_3和P_4。
Molecular techniques, such as plasmid profiles, have also been of limited value in identifying individual strains of Vibrio cholerae.
在个别霍乱弧菌的菌株鑑定上，分子生物技术如质体图谱的价值也是有限的。
The effects of plasmid pcDNA3 on Ca2+ transport of sarcoplasmic reticulum (SR) in ischemic skeletal muscle was investigated.
在大鼠肢体缺血模型上观察了质粒pcDNA3对缺血骨骼肌肌浆网(SR) Ca2+转运的影响。
In our previous work, we contructed a recombinant plasmid pBV-SBP and expressed recombinant SBP in E.coli.
在本室前期的研究工作中，我们构建了原核表达载体pBV-SBP，用E.coli大量表达重组SBP。
The traditional alkaline method to isolate plasmid DNA takes time and the operation system requires strict control.
实验室中常用的质粒提取方法是碱裂解法。
The results of experiment showed that B. subtilis BR151 and MI112 containing plasmid pUB110 in LB liquid medium is more stabl than in LB slant.
实验证明枯草芽孢杆菌(Bacillus subtilis)BR151和MI112中的pUB110质粒,在液体中传代要比固体斜面传代稳定。
The factors influencing survival rate of LAB and plasmid stability during lyophilization were systematically studied.
对乳酸菌冻干存活率的影响因素及质粒稳定性进行研究。
The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body.
对含人EC-SOD重组质粒的工程菌进行诱导表达形成包涵体。
Routine alkaline lysis miniprep of plasmid DNA was modified,and LiCl was specially used.
对碱裂质粒小量法进行了改进，并且在提取过程中增加了LiCl处理。
The sulfonamides, streptomycin, chloramphenicol and other sensitive, but easy to resistance, with R factor is the transfer of plasmid obtained.
对磺胺类、链霉素、氯霉素等敏感，但易耐药，是由带有R因子的质粒转移而获得的。
Recombinant plasmid pDAlllS was constructed by inserting 1.7kb aprA into the Nrul site of aveD.
将1.7kb安普霉素抗性基因aprA插入aveD的NruI位点，得pDA1118。
The hTR gene was then reverse inverted into retrovirus vector pLNCX and constructed an antisense recombinant plasmid pLNCX-aTR.
将hTR基因插入逆转录病毒载体pLNCX中，构建了hTR基因的反义重组质粒pLNCX-aTR和正义重组质粒pLNCX-TR，测序结果表明符合预期要求，为下一步实验奠定了基础。
First, the two genes were cloned into shuffle vector pFastBac Dual one after another. The recombinant plasmid was named as pBD-CDl- 2m.
将mCDld和pZm先后连入穿梭质粒pFastBac Dual，获得重组质粒pBD一CDI一pZm;
To transform PET-BPI193 recombinant plasmid into E.coli BL21 and 6His-BPI193 fusion protein was expressed by IPTG induced manner.
将PET-BPI193重组表达质粒转化入大肠杆菌BL21，通过IPTG诱导，获得了BPI193与组氨酸融合表达的重组蛋白。
Construction of pFASTBacHTb - HPV6L1 donor plasmidThe cloned plasmid on vector pGEM - T was digested with BamHI and Eco-Rl.
将PGEM－T载体上的克隆质粒经BamHI和EcoRI酶切后，与经Burn－HI禾 EcoRI酶切后的 pFASTBacHTb质粒连接，构建p以STBacHTb－HPV6LI供体质粒。
Purified ATF-lys10 specifically bound to uPAR-expressing cells and formed protein-DNA complexes with plasmid pGL3-control.
将uPAR反义核酸表达质粒与ATF一lyslo以及多聚赖氨酸组装成基因转移复合物，并转染95D细胞。
The experiment results covered several following points:(1) adw was cloned into nuclear plasmid PB, PBG and chloroplast expression plasmid PLCTB .
将乙肝表面抗原基因(adw型)与细胞核载体PB、PBG以及叶绿体表达载体PLCTB进行定向克隆，经PCR和测序鉴定都获得了重组子，分别命名为PB-adw、PBG-adw、PLCTB-adw。
The VH and VL genes were inserted into a plasmid which contains a linker sequence for constructing scFv gene.The new vector named pTC scFv.
将其和先前克隆的轻链基因分别插入到一个含有连接VH和VL基因的连接序列的质粒之中，构建成单链抗体（scFv）基因的表达载体pTCscFv。
Expression and purification of recombinant RTAsThe expression plasmid Pkk223.3-RTA was introduced into E. coli JM 109 by CaCl2-mediated method.
将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-YQRL转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-YQRL蛋白。
The GRF gene plasmid residue didn’t be found in a few of organs injected calves after slaughter.
屠宰后进行组织质粒残留检测，未发现有残留。
Switch on the vacuum to draw the solution through the column (The plasmid DNA binds to the column material).
开启真空装置使液体通过管柱(质体DNA会与管柱物质结合)。
STUDY ON PLASMID FINGERPRINTING AND ANTIBIOTIC SUSCEPTIBILITIES OF SHIGELLA SPP.
志贺菌流行株药物敏感性及质粒图谱分析。
The recombinant CYP3A4 plasmid was successfully cloned and effectively expressed in Rosetta(DE3)2pLysS.
成功地使CYP3A4基因在原核系统中高效表达.
The recombinant plasmid pBV220-Arr was construted successfully and the target protein Arresten can express in E.
成功构建Arresten基因重组质粒pBV220-Arr,并可在E.
The plasmid pBV220-Arr. could be expressed in E.
成功构建的重组质粒pBV220-Arr在E.
The recombinant eukaryotic expression plasmid,pcDNA3.1A DCN was successfully constructed and 2 cell clones positively expressing DCN were selected.
成功构建重组真核表达质粒 pcDNA3 .1A DCN ,转染MsC并筛选出 2个阳性克隆株。
The Arresten gene was screening successfully,and the recombinant plasmid pBV220-Arr was constructed successful.
成功筛选出Arresten基因并构建了重组质粒pBV220-Arr,重组质粒在菌株中获得表达。
GUS,35S,hpt and aadA gene of plasmid pCAMBIAlSOl are simultaneously specifically detected by using arryed primer extension chip.
我们利用此种芯片实现了对质粒pCAMBIA1301中所含的四个基因GUS、35S启动子、hpt、aadA的特异性同步检测。
We explored four types of repeats in 58 fully sequenced bacterial genomes, including 60 chromosomal and 22 plasmid sequences.
我们探讨了在58个已被完整定序的细菌基因体（包含了60个染色体序列及22个质体序列）之中出现的四种重覆序列。
We have observed, in our previous report, that NF IL6 can act as a tranforming gene when cotransfected with pSV2 neo plasmid.
我们曾以pSV2?neo作为共转染质粒观察到NF?IL6具有转化基因活性。
P16-promoter-luc-pGL2-Basic plasmid DNA was prepared after plasmid amplification, and digested by EcoR I and Hind III.
扩增p 16 promoter一luc一pGLZ一Basic报告基因表达质粒并提取其DNA。
Transfer of a segment of DNA to a new position on the same or another chromosome, plasmid, or cell.
换位把脱氧核糖核酸的一个片断转移到同一个或另外的染色体、质体或细胞上的一个新位置上
Therefore we consider that th e pock formation ability in S.qingfengmyceticus may be determined by plasmid gen e (or genes).
据此，我们认为庆丰链霉菌中产生麻点的能力很可能由质粒基因所决 定。
This P element then transposes from the plasmid to a random chromosomal site.
携带目的基因的P因子可从质粒转座到任意染色体上。
A simpler mathematical model with less assumption to measure plasmid loss rate is developed in this study.
摘要本研究利用简单的数学模式与较少的假设推导质体流失率。
Objective To control the quality of mouse nerve growth factor (NGF) DNA plasmid for gene therapy.
摘要目的对小鼠神经生长因子(NGF)基因治疗型DNA质粒进行质量控制。
Objective To develop a procedure for large-scale purification of plasmid DNA.
摘要目的建立适合大规模纯化质粒DNA的工艺。
Obejective: To invesitigate the plasmid and drug resistance strains in 150 strains Escherichia coli isolated from healthy person in ShenZhen city.
摘要目的：对深圳地区健康人肠道分离的150株大肠埃希菌进行质粒图谱分析，探讨质粒谱与耐药性的关系。