endonuclease单词基本解析:
n.核酸内切酶n. [生化]核酸内切酶
n.核酸内切酶;
endonuclease变化用词:
endonuclease英英释义:
Noun1. a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes
名词 endonuclease:
a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes
endonuclease[ ,endəu'nju:klieis ]n.a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes
endonuclease中文词源:
endonuclease用法和例句:
Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.
方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法.
互联网
endonuclease
A portion of Ligation products were identified by BamHI and Hin-dIII restriction endonuclease and was named pUC - B2 - AR.
2·PMCX-p。 -AR反义表达载体的酶切鉴定,其结果与理论设计完全相符。
AP endonuclease 1
AP内切酶1
The ERG 11 gene of all isolates had been amplified successfully. There were some differences on both the Ace I and the Mun I endonuclease maps of ERG 11 gene between the hyphal form and the yeast form of C. albicans.
CA-7、CA-14,CA-16和CA-17的菌丝相与酵母相ERG11基因酶切图谱存在差异;
Nicking Endonuclease
Nicking核酸内切酶
the PCR products were screened mutations using restriction endonuclease fingerprinting-single strand conformation polymorphism(REF-SSCP);
PCR产物通过限制性酶切-单链构象多态性方法检测未知突变;
After the PCR amplicons of SEE strain sere eleaved by restriction endonuclease EcoRV.electrophofretic analysis showed two 251 and 415bp DNA fragments.
SEE菌株的扩增产物经EcoRV酶切能产生251和415bp两个片段。
T4 Endonuclease V
UV-B辐射
Setting Up a Restriction Endonuclease Reaction
上一篇:酶切反应
Keywords Hepatitis C virus(HCV);Genotype;Interferon(IFN);Polymerase chain reaction (PCR);Restriction endonuclease;
丙肝病毒;基因型;干扰素;聚合酶链反应;限制性内切酶;
Using recombination techniques, the gene fragment CED-9, derived from pBS, was incorporated into pGEM-7zf(+) and formed a new vector pGEM-ced9, in order to get the right endonuclease sites.
从质粒pBS中获取CED-9基因片段,构建中间载体pGEM-ced9以获得合适的酶切位点。
Uses advanced biotechnology to simulate the T4 Endonuclease produced by natural marine algae.This helps to repair and restore damaged DNA cells in the skin and erase fine lines and wrinkles.
以先进科技模拟天然海藻中具修补功能的T4酵素,重建受损DNA,杜绝幼纹、皱纹的出现。
The study on restriction endonuclease enzyme analysis method in atypical pneumonia
传染性非典型肺炎限制性内切酶检测方法的研究
deoxyribonucleic acid repair endonuclease
修复DNA的内切核酶
repair endonuclease
修复核酸内切酶
Whole Genome Amplification Technology, Mutagenic Endonuclease Restriction Assays, and Their Expanding Applications in Molecular Diagnostics
全基因组扩增技术,致突变的核酸内切酶限制性分析,及其在分子诊断中的广阔应用
Keywords Hairpin fluorescence molecular probe;DNA;DNA methylation;Methylase;Restriction Endonuclease;
关键词发夹型荧光分子探针;DNA;DNA甲基化;甲基化酶;限制性内切酶;
intron encoded endonuclease
内含子编码内切核酸酶
apoptotic endonuclease
凋亡内切酶
apoptosis-specific endonuclease
凋亡特异核酸酶
Standardization of restriction endonuclease expression in molecular biology books and periodicals
分子生物学书刊中限制性内切酶的规范表达
Single-stranded-nucleate endonuclease
单链核酸内核酸酶
isoschizomer-heterotail restriction endonuclease (IHRE)
同序异尾限制性内切酶(IHRE)
Experimental identification of endonuclease activity of the putative gene tls from phage PaP3
噬菌体PaP3推定基因tls核酸内切酶活性的初步验证
Newcastle Disease Detection by Gene Chip and Typing of Newcastle Disease Virus by RT-PCR Test Coupled with Restriction Endonuclease Analysis
基因芯片检测新城疫病毒和PCR结合内切酶分析鉴别新城疫强弱毒株的研究
Keywords multiple myeloma;apurinic/apyrimidinic endonuclease;DNA damage repair;
多发性骨髓瘤;脱嘌呤/脱嘧啶核酸内切酶;DNA损伤修复;
restriction endonuclease from e. coli
大肠杆菌限制性核酸内切酶ⅰ, 大肠杆菌限制性核酸内切酶ⅱ
RESTRICTION ENDONUCLEASE FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF MITOCHONDRIA DNA IN EGGS OF BOMBYX MORI
家蚕卵线粒体DNA限制性内切酶长度多态性研究
The PCR products were cloned into T clone vector,and the positive clones were picked out,then the T clone vector was identified by restriction endonuclease digestion.
将扩增的连接产物克隆入T载体,挑选阳性克隆并进行酶切鉴定,酶切鉴定正确的克隆进行拼接产物的核苷酸序列测序。
RESTRICTION ENDONUCLEASE ANALYSIS OF CYTOMEGALOVIRUS STRAINS ISOLATED FROM CHILDREN ATTENDING KINDERGARTENS
幼儿园儿童巨细胞病毒分离毒株的限制性酶切图谱分析
PCR amplification and restriction endonuclease digestion was used to identify deleted DNA fragments.
应用PCR技术与限制性内切酶酶切相结合的方法鉴定缺失子。
The 344C/T polymorphism of CYP11B2 gene was monitored by PCR and HaeIII restriction endonuclease digestion methods.
应用多聚酶链式反应(PCR)、限制性内切酶方法检测CYP11B2基因的多态性分布。
Keywords Toxoplasma gondii;GRA7 gene;restriction endonuclease;clone;
弓形虫;GRA7基因;限制性内切酶;克隆;
homing endonuclease
归巢内切酶
micrococcal endonuclease
微球菌核酸内切酶
Keywords Shigella;Plasmid profile;Restriction endonuclease pattern;RAPD-PCR;Shigella enterotoxin;
志贺氏菌;质粒图谱;限制性内切酶图谱;随机PCR;志贺氏肠毒素;
Torres into the first ball is from the edge of Endonuclease Houweiebei de Xiadichuanzhong assists.
托雷斯所进第一球,正是来自边后卫阿贝罗阿的内切下底传中助攻。
damage specific endonuclease
损伤特异性内切酶
Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.
方法用盐提取法提取人群中白细胞的DNA,以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。
Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.
方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
Method The two clones of OSCP gene were modified by restriction endonuclease and recombined by T_4DNA ligase.
方法通过限制性核酸内切酶将发生有义突变的两个OSCP基因的DNA克隆进行改造,并进行基因重组。
The purpose of the experiment is to construct pcDNA3 expression vectors containing p27 gene by PCR and endonuclease lysis.
本实验的目的在于通过PCR、酶切等步骤构建含有p27基因的载体pcDNA3,然后通过脂质体转染进入肿瘤细胞MCF7中,筛选到稳定表达株。
5 Restriction endonuclease map of MmPDV geomeM is molecular weight marker;
标题: 图5 MmPDV基因组不同内切酶图谱(M.分子量标准;
nucleate endonuclease
核酸内切酶
endonuclease recognition site
核酸内切酶, 识别位点
retriction endonuclease
核酸内切限制酶
restricted endonuclease
核酸内切限制酶, 限制性核酸内切酶
A ,sad sequence ;B ,restriction endonuclease cutting sites of sad.
段,与序列分析结果相一致(图略)。
Keywords chlamydia trachomatis;polymerase chain reaction;hybridization;restricted endonuclease;
沙眼衣原体;聚合酶链反应;杂交法;限制性内切酶;
Keywords Japanese encephalitis virus;recombinant plasmid;DNA sequencing;restriction endonuclease analysis;
流行性乙型脑炎病毒;重组质粒;DNA测序;限制性内切酶分析;
Keywords Neisseria gonorrhoeae;Resistant plasmids;Restriction endonuclease;
淋病奈瑟球菌;耐药质粒;限制性核酸内切酶;
flap endonuclease
激动内切核酸酶
Secondly,the preamplified DNA fragments were digested by a restriction endonuclease to form sticky ends,which were then ligated to a designed DNA adapter by ligase.
然后用限制性内切酶将其消化成短片段,在连接酶的作用下与设计的DNA适配器相连;
The Extraction and Purification of Restriction Endonuclease from Local Moraxella bovine Strain of China
牛摩拉氏菌中国地方株限制性核酸内切酶的提取与纯化
Analysis of ethambutol drug-resistance gene mutation in Mycobacterium tuberculosis by specific endonuclease
特异性核酸内切酶检测结核分枝杆菌的乙胺丁醇耐药基因突变
The OmpL1 gene of Leptospira(L.) serovar lai was amplified and sequenced, and its nucleotide sequence, protein secondary structure and restriction endonuclease map were further analysed.
用PCR方法扩增不同毒力赖型钩体OmpL1基因片段,进行序列测定,用相关软件比较分析核苷酸序列、蛋白质二级结构以及限制性内切酶谱。
AIM: To explore the mechanism of neuronal injury and repair by investigating the expression of caspase-3 and apurinic/apyrimidinic endonuclease (APE/Ref-1) after focal cerebral ischemia.
目的 :观察脑缺血后半胱氨酸蛋白酶 3 (caspase - 3 )和脱嘌呤 /脱嘧啶核酸内切酶 (APE/Ref- 1)的表达 ,探讨脑缺血损伤与修复机制。
minor endonuclease
稀切酶
ultraviolet endonuclease
紫外内切核酸酶
After identification with restriction endonuclease digestion, sequencing, and indirect immunofluorescence (IF), the suicidal DNA vaccine (pAHSP65) was inoculated into mice.
经酶切、测序鉴定,间接免疫荧光(IF)证实其能表达HSP65后,将此“自杀性”DNA疫苗(pAHSP65)免疫小鼠。
Mimics of both types of BCR ABL cDNA were achieved and the validity was verified with restriction endonuclease.
经酶切分析证明 ,两型BCR ABLmRNA均可通过此方法得到相应的cDNA参照物。
Restriction endonuclease digestive identification was right for recombinant expression vector pCD11b BFP. Blue fluorescence from fusion protein could be seen in U937 cells transfected with plasmid pCD11b BFP.
经酶切鉴定 ,p CD11b- BFP构建完全正确 ,转染 U937细胞株后 ,可见 CD11b- BFP融合蛋白发出的蓝色荧光。
Results The result of restriction endonuclease digestion was accordance with the anticipated objective strap size .
结果 酶切结果与预期目的条带大小相符;
Results:The constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.
结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45-4B基因。
Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful.
结果酶切及测序显示对OSCP基因有义突变部分的纠正获得成功。
Conclusion Increase in cytoplasmic calcium activates endonuclease(s) which causes DNA fragmentation at internucleosomal sites and neuronal apoptosis.
结论 胞浆钙离子升高激活内源性核酸内切酶,导致DNA核小体间断裂,启动神经细胞凋亡。
Conclusion The plasmid DNA restricition endonuclease analysis was more specific and sensitive than plasmid profile, but they both had some restrictions.
结论质粒分析能较为特异、敏感地揭示不同来源志贺菌间的遗传联系和差别,从而准确说明爆发或流行事件的真相。
The Detection of Human Cytomegalovirus in Gravida with the Early Trimester in Pregnancy and Neonates by Nested Polymerase Chain Reaction with Restriction Endonuclease and Virus Isolation
聚合酶链反应限制酶切分析及病毒分离检测巨细胞病毒感染
Keywords cerebral ischemia;reperfusion;gene expression;endonuclease;
脑缺血;再灌注;基因表达;核酸内切酶;
apurinic/apyrimidinic endonuclease
脱嘌呤/脱嘧啶核酸内切酶
Keywords apurinic/apyrimidinic endonuclease 1;colorectal neoplasms;gene expression;
脱嘌呤/脱嘧啶核酸内切酶;结直肠肿瘤;基因表达;
apurinic endonuclease
脱嘌呤内切核酸酶
AP endonuclease
脱嘌呤嘧啶内切核酸酶
apurinic-apyrimidinic endonuclease
脱嘌呤嘧啶核酸内切酶
Apurinic/Apyrimidinic Endonuclease 1
脱嘌呤/脱嘧啶核酸内切酶
Apurinc/apyrimidinic endonuclease 1
脱嘌呤/脱嘧啶核酸内切酶1
Apurinic / apyrimidinic endonuclease / redox factor-1
脱嘌呤/脱嘧啶核酸内切酶/氧化还原因子-1
deoxyinosine endonuclease
脱氧肌苷内切核酸酶
MOLECULAR CLONING AND RESTRICTION ENDONUCLEASE ANALYSIS OF Euproctis pseudoconspera NUCLEAR POLYHEDROSIS VIRUS DNA
茶毛虫核型多角体病毒基因组酶切分析及质粒文库的构建
Recombinant vector of pUCm-T/mIA-2i-mIgG Fc was prepared by gene combination and confirmed by PCR and dual-site endonuclease.
获得重组克隆质粒pUCm-T/mIA-2i-mIgGFc,经聚合酶链反应和双位点酶切鉴定重组成功。
Keywords plasmid;cyanide;restriction endonuclease;gram negative bacteria;
质粒;氰化物;限制性内切酶;革兰氏阴性细菌;