electroporation是什么意思   electroporation怎么读

electroporation单词基本解析：

electroporation变化用词：

electroporation英英释义：

electroporation中文词源：

electroporation用法和例句：

electroporation

Keywords tri-parental mating;electroporation;gene transfer;T-DNA;Agrobacterium tumefaciens LBA4404;PCR;

三亲杂交;电击转化;基因转移;转移DNA;根癌农杆菌LBA4404;多聚酶链式反应;

Keywords josamycin;Breeding;titer;Media;screen;mortality;mutate;electroporation;

交沙霉素;育种;效价;培养基;筛选;抗性质粒电转化;

Electroporation and its Mechanism due to Low Amplitude Transient Electromagnetic Pulsess on Cell Membranes

低强度瞬态电磁脉冲对细胞膜的电穿孔效应及机理研究

In vivo electroporation

体内电穿孔

That electroporation can induce apoptosis is investigated in HeLa cells, the effects of electroporation to apoptosis of HeLa cells is studied with Giemsa,Acridine orange,Hoechst33342 and propidium iodide staining.

作者报道了电穿孔可以诱发HeLa细胞凋亡 ,采用Giemsa染料、吖啶橙染色及Hoechst33342 ,PI双染的荧光染色 ,研究了电穿孔对HeLa细胞凋亡的影响 .

Keywords T4 lysozyme;Pichia pastoris;Electroporation;Expression;Antibacterial activity;

关键词T4溶菌酶;毕赤酵母;电击转化;表达;抗菌活性;

Keywords Electric stimulation therapy;Combined modality therapy;Electroporation;Neoplasms;

关键词电刺激疗法;综合疗法;电穿孔;肿瘤;

Keywords electroporation transfection;chlamydia trachomatis;vector;

关键词电穿孔;沙眼衣原体;载体;

Keywords Electroporation Electrochemotherapy（ECT） S 180 Sarcomas Anticancer drug;

关键词电穿孔;电化学疗法（ECT）;S-180肉瘤细胞;抗癌药物;

Keywords telomerase;telomeraseRNA;antisense;electroporation;retroviral vector;pancreatic cells;

关键词端粒酶;端粒酶RNA;反义;电穿孔;逆转录病毒载体;胰腺癌细胞;

Keywords Steep pulse;Electroporation;Irreversible electrical breakdown;Malignant tumor cell;

关键词陡脉冲;电穿孔;不可逆性电击穿;恶性肿瘤细胞;

Keywords endostatin;electroporation;gene transfer;tumor growth;

内抑素;电穿孔;基因转移;肿瘤生长;

Using calcein as an indicator, the effects of electroporation on de exined pollen, ungerminated and germinated intact pollen in Nicotiana tabacum were compared.

利用膜不通透的荧光染料钙黄素作为指示剂，比较了烟草脱外壁花粉与完整的未萌发花粉和萌发花粉电激导入的效果。

Keywords BCG;ERP gene;Gene knockout;electroporation;

卡介苗;ERP基因;基因敲除;电穿孔;

Keywords Denervation;Muscular atrophy;Electroporation;Gene transfer;

去神经支配;肌萎缩;电穿孔;基因转染;

In-situ electroporation of cells in the dish or on a slide.

在培养皿或玻片上细胞的原位电穿孔

Electroporation has been widely used to manipulate cells.Both applications and mechanistic understanding have received growing attention over the past two decades.

在过去的20年,电穿孔技术的机理和应用受到了很大的关注,而且细胞电穿孔是一种生物工程新技术。

Research on the Voltage-controlled Electroporation Model Exposed to External Electrical Field

外电场作用下压控电穿孔模型的研究

Parameters of electroporation in transgene of Pinctada maxima

大珠母贝基因转移的电击参数

Keywords Protoplasts of barley;Electrofusion;Electroporation;Membrane adsorption;Fluorescent dextra;

大麦原生质体;细胞电融合;电生孔;膜吸附;荧光葡聚糖;

in utero electroporation

子宫内电转染

After the recombinant plasmid pMET-PLF-N was linearized and transformed into PMAD11 compentent cells by electroporation, Ade+(Muts)recombinants were selected and induced with methanol.

将此重组表达载体线性化后通过电转化的方法转化PMAD11酵母感受态细胞，筛选Ade~+(Mut~s)重组子并诱导表达。

Finally, The recombinant plasmid pVAX1-S1 was transformed by electroporation into attenuated Salmonella typhimurium strain SL7207 and confirmed by PCR and Salmonella agglutination test. The recombinant was named as SL7207(pVAX1-S1).

将重组质粒转入减毒鼠伤寒沙门氏菌SL7207中,构建出运送DNA疫苗的重组沙门氏菌SL7207(pVAX1-S1)。

Keywords wheat;transformation by biolistic method;snowdrop lectin（Galanthus nivalis agglutinin,GNA）;aphid;resistance;transformation by electroporation;ring electrode;immature embryo;

小麦;基因枪转化;雪花莲凝集素;蚜虫;抗性;电激转化;环形电极;幼胚;

This review gives an overview of the application, effectiveness and prospect of electroporation in improving the efficacy of DNA vaccine...

就电穿孔技术在核酸疫苗研究领域的应用作一综述。

Y Wang， C J Liu， Chang Hong，et al．Membrane Electroporation Due to Weak Transient Electromagnetic Pulses[R]．The 4TH Asis-Pacific Conference on Medical and Biological Engineering．1999， 9．Seoul, Korea

张弘，王保义，刘长军，等．利用弱电磁脉冲对细胞电穿孔提高抗癌药物疗效的研究[J]．电波科学学报，1999，14（增刊）：397-400

Keywords Gene therapy;Insulin;Electroporation;Skeletal muscle;Diabetes mellitus;Doxycycline;

强力霉素;电穿孔;基因治疗;骨骼肌;糖尿病;胰岛素;

The Experimental Study of Electroporation of Cell Membrane Induced by Pulsed Electric Field of High Intensity

强脉冲电场致细胞膜电穿孔的实验研究

Our study shows that intratumoral electroporation of interleukin 12 genes suppressed tumor growth in mice and canine models, and a phase I clinical trial is designed to treat cancer patients.

我们的研究也证明经由肌肉电击传送介白素-12，能有效抑制小鼠和狗的肿瘤生长，即将对癌症病人进行第一期的临床试验。

FDR3 was inserted into the yeast expression vector pDBLeu and then was transferred to the yeast strain M3(ctrl mutant) mediated by PEG/LiAc and by electroporation .

把FDR3基因片段克隆到酵母表达载体pDBLeu上，然后通过电击和乙酸锂转化两种方法将重组质粒导入酿酒酵母Ctrl突变体（M3 mutant）中，采用酵母异源互补法进一步鉴定FDR3基因的功能。

This paper briefly reviewed some useful gene transfer techniques, including microinjection, liposome-mediated, electroporation, ES-mediated, virus-mediated, sperm-mediated and ovary-injection.

摘要简要介绍了几种比较常用的转基因方法，包括显微注射法、脂质体介导法、电转染法、胚胎干细胞法、病毒载体法、精子载体法、卵巢直接注射法。

Methods:NPC cell line (CNE1) as target,Electroporation was used to transfect EBV-LMP gene and the vector into CNE1 cells.

方法：以人高分化鼻咽癌细胞株(CNE1)为对象，采用电穿孔基因转染技术，将重组EBV-LMP表达质粒转染CNE1细胞。

Keywords Schistosoma japonicum;Electroporation;Fluorescent protein gene;Transgenes;Gene expression;

日本血吸虫;电穿孔;荧光蛋白基因;转基因;基因表达;

Electroporation refers to the use of electro-osmosis and the principle of non-invasive painless to import active ingredients or nutrition to beauty therapy skin.

是指利用电穿孔和电渗透的原理，无创无痛的把活性成份或营养导入到皮下的美容疗法。

This work systematically investigated the conditions of the electroporation on the plasmolemma of Saccharomyces ccrevisiae Hahsen Var ellipsoideus Dekker (Nan-Yang No. 6 "K" ).

本实验系统地研究了椭圆酿酒酵母AS2-607(南阳6号“K”)质膜的电穿孔条件。

The characteristic of an electroporation apparatus for transdermal drug delivery is discussed in the paper.

本文分析了一种经皮给药电穿孔仪的性能。

Themechanisms,experimental devices and the influence factors of electroporation are reviewed.The application and the safetyof the electroporation in transdermal drug delivery are also introduced.

本文对电致孔法的透皮促渗机制、影响因素以及安全性进行了讨论,并介绍了有关实验装置及其在透皮给药中的应用。

We studied a way of electroporation with low intensive for TDD by the energy of the electroporaton on drug's transdermal flux.

本文研究了电穿孔能量对盐酸丁卡因和萘普生经皮渗透速率的影响，探索低压电穿孔技术的途径。

The methods include using Agr obacterium-mediated transformation, electroporation, gene gun bombardme nt and polyethyleneglycol (PEG)-mediated direct DNA transfer.

果树基因转化方法主要包括农杆菌介导法、电激法、基因枪法、PEG转化法等。

Keywords neucleic acid immunity;hemorrhagic fever renal syndrome virus（HFRSV）;eukaryotic expressing vector;electroporation;transient expressing;

核酸免疫;肾综合征出血热病毒;真核表达载体;电穿孔;瞬间表达;

Keywords Immunoglobulin genes;rat monoclonal antibody;HTV;single chain antibody;chimeric antibody;electroporation;J558L cell;HFRS;phage display;

汉滩病毒;肾综合症出血热;大鼠单克隆抗体;可变区基因;嵌合抗体;单链抗体;噬菌体展示;

In Vitro Culture of Bovine Fetal Fibroblast Cells Using Tissue Explant Attachment and Gene Transfection Through Electroporation

牛胎儿成纤维细胞的组织块贴附法分离培养与电穿孔法基因转染

Electroporation competence

电击转化

Keywords electro-chemotherapy （ECT）;electric field distribution;electroporation;model;Finite element method;Analytics method;

电化学疗法;电场分布;电穿孔;模型;有限元法;解析法;

Keywords Electrochemotherapy;Electroporation;Tumor treatment;Needle electrode;

电化学疗法;电穿孔;肿瘤治疗;针电极;

Keywords electrical injury;electroporation;pathophsiology;pathogenesis;

电流损伤;病理生理学;发生机制;

Keywords electroporation;ring electrode;transformation;wheat;immature embryo;

电激;环形电极;转化;小麦;幼胚;

Keywords electroporation;cell cultured in suspension;gene transfection;optimization;

电穿孔;悬浮细胞;基因转染;优化;

Keywords electroporation;electric pulse;electrochemotherapy;tumor treatment;electric field distribution;mechanism analysis;

电穿孔;电脉冲;电化学疗法;肿瘤治疗;电场分布;机理分析;

Keywords electroporation;transformation;transformation efficiency;

电穿孔;转化;转化效率;

Delaying of denervated muscle atrophy by means of gene transfer of ciliary neurotrophic factor by electroporation

电穿孔介导睫状神经营养因子基因转染延缓失神经肌萎缩

electroporation apparatus

电穿孔仪

electroporation cuvette

电穿孔小槽

APPLICATION OF ELECTROPORATION TO TRANSFECTING OF EBV-LMP EXPRESSING PLASMID INTO NPC CNE1 CELLS IN VITRO

电穿孔技术在EBV-LMP表达质粒转染人鼻咽癌细胞中的应用

Application of Electroporation in Gene Transfer and Animal Embryo Cloning

电穿孔技术在转基因及动物克隆中的应用

Electroporation is a biophysical phenomenon referring to the formation of transient reversible micro-pores on the phospholipid bilayer of cell membrane under the transient electric field.

电穿孔是指在瞬态电场作用下,瞬时增强细胞膜的通透性,在细胞膜的双脂层上形成瞬时可逆微孔的生物物理现象。

electroporation buffer

电穿孔缓冲液

The application and the safetyof the electroporation in transdermal drug delivery are also introduced.

电致孔可显著提高药物的经皮吸收,有望用于多肽和蛋白质类生物大分子药物的透皮给药。

Keywords electroporation;chick embryo;skeletal development;schizophrenia;single nucleotide polymorphism;association study;

电转化;鸡胚;骨骼发育;精神分裂症;单核苷酸多态性;关联分析;

In order to overcome this barrier, several chemical and physical methods have been developed, such as electroporation and cationic lipids/liposomes.

目前流行的入胞转运方式如电穿孔、脂质体转染等均存在对细胞的额外毒副作用，且作用范围局限于体外实验。

Recently, novel delivery systems, especially electroporation, have overcome this translational block.

目前，新的递药系统，尤其是电穿孔法，已经克服了传统的阻碍。

Objective To optimize the transfection rate in COS 7 cell line with electroporation method.

目的 以电穿孔法转染COS 7细胞系以得到较高的转染率。

Keywords seminiferous tubule injection;electroporation;transgenic mice;

睾丸生精小管内注射;体内电穿孔;转基因小鼠;

Insulin-like growth factor;electroporation;Candida utilis;transformant;bioactivity

类胰岛素成长因子;电转形;饲料酵母;转形株;生物活性

Keywords Cell density;Electroporation;Vitality of cell;Transmembrane voltage;

细胞浓度;电穿孔;细胞活力;膜电压;

immunity cell electroporation

细胞电生孔

Elementary Exploration of the Mechanism and Applicable Prospects of Membrane Electroporation

细胞膜电穿孔的机理及应用前景的初步探讨

Keywords transdermal drug delivery;electroporation;truncation coefficient;transdermal energy;pulse protocol;

经皮给药;电穿孔;截断系数;经皮能量;脉冲选择;

Keywords transdermal delivery;electroporation;Tween 80;passive diffusion;piroxicam;

经皮给药;电致孔;聚山梨酯80;被动扩散;吡罗昔康;

Development of Electroporation Apparatus for Transdermal Drug Delivery

经皮给药电穿孔仪的研制

Accordingly,the electroporation can enhance the cytotoxicity of antitumor drug.This is a piece of new approach for antitumor.

结果说明,利用电穿孔可提高抗肿瘤药物的细胞毒性,为治疗肿瘤提供了新的途径。

Conclusion: The combination of electroporation and the patch can improve the speed and extent of transdermal absorption of sinomenine, and thus enhance the bioavailability.

结论: 将电致孔技术与巴布剂结合能够提高药物透皮吸收的速度和程度，从而提高生物利用度。

The transdermal delivery of piroxicam was pronouncelly improved by enhancing the solubility of piroxicam with Tween 80 and forming LTRs by electroporation.

综合利用聚山梨酯80的促渗作用和电致孔时形成的水性通道,可以有效提高吡罗昔康的透皮量。

Since 1983, demanding researchers worldwide have made BTX products their choice for critical electroporation and electrofusion applications.

美国BTX公司自1983年成立以来，以电穿孔仪、电融合仪为主要产品。

Growth styles and rules of myoblasts of newborn rats afer arenzymedigestion as well as the damage of simulated electroporation stimulation in gene transfection to the cryopreservation and resuscitation of cells

胰酶消化后新生鼠成肌细胞生长方式与规律以及模拟转基因电穿刺激对冻存和复苏细胞的损害

Keywords Candida Cloacae;fatty-acid alcohol oxidase gene;vector construction;electroporation;

脂肪酸醇氧化酶基因;载体构建;电击转化;

Keywords Giardia lamblia virus;electroporation;GFP;ELISA;

蓝氏贾第虫病毒;电穿孔;绿色荧光蛋白;ELISA;

Keywords Tansgenic Schistosome;EGFP;Electroporation;Transfection;Lipofectamine;

转基因血吸虫;EGFP;电穿孔;转染;脂质体;

The Method of in vivo Gene Transfection into Mouse Brain by Means of in Utero Electroporation

运用电穿孔技术建立小鼠脑内基因转染模型

Two types of vectors are commonly used, each of which can be introduced by electroporation or retroviral infection.

通常采用外显子陷阱和内含了陷阱两类。